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Research Core Facilities. The School of Medicine supports
several state-of-the-art core facilities that are available to
investigators on a fee-for-service basis. These facilities are
administered by Jay W. Fox, Ph.D., Assistant Dean for Research Support,
and an advisory committee. Each core is overseen by a faculty
committee; the SOM Research Advisory Committee (RAC) recommends annual
subsidies to the Dean.
- Advanced Microscopy Facility. The Advanced
Microscopy Core (AMC) provided expertise, training and instrumentation
associated with confocal and electron microscopy (TEM and SEM). Sample
preparation and operation of core instrumentation can be performed by
core staff or trained users.
- Biomolecular Research Facility. The Biomolecular
Research Facility is divided into two program areas: Mass
spectrometry/Proteomics and DNA
Sciences.
The Mass Spectrometry/Proteomics area supports experimentation in
protein identification, post-translational modification analysis,
binding partner/complex mixture analysis and quantitative mass
spectrometry utilizing iTRAQ and SILAC approaches. The DNA Sciences
Laboratory offers gene expression profiling by Affymetrix platforms,
genomic analysis using a Solexa analyzer and DNA sequencing.
- BioNMR Core (Contact Jeff Ellena, Laboratory Director or
Dr. John
Bushweller, Faculty Director). The BioNMR Core has two NMR
spectrometers (600MHz and 800MHz) for use by trained investigators or
core staff. Expert consultation in molecular structural elucidation and
molecular dynamics is available.
- Biorepository and Tissue Research Facility. This resource provides prospective and banked
human tissue procurement; processing and storage of all types of
biospecimens including blood, urine and tissues; enzymatic dissociation
and freezing of tissue samples; and extraction of nucleic acids and
proteins from tissues. Laser microdissection, immunohistochemistry and
tissue microarray production are also available.
- Flow Cytometry Core Facility. The core maintains a variety of cell sorters and
analyzers for state of the art cellular characterization. Expert
consultation is available for experimental design and instrumentation
is available for use by trained investigators or experiments can be
performed by the staff. Several training/educational programs are
offered throughout the year for investigators, students and staff.
- Gene Targeting and Transgenic Facility. The range
of services offered by the GTTF include genetic engineering of mutant
mice; genetic analysis of mutant mouse strains and cryopreservation of
mutant lines. The facility offers a yearly educational workshop to
train investigators and staff in technologies associated with genetic
manipulation of mice.
- Lymphocyte Culture Center. The primary function of
this core is to construct and select lymphocyte-myeloma hybridomas for
the production and purification of monoclonal antibody reagents as well
as expert consultation on experimental design leading to monoclonal
production and antibody
characterization.
- Molecular Imaging Core. The aim of the core is
to provide noninvasive monitoring of biological processes in vivo using
a variety of anatomical and functional imaging modalities. Additionally
development of targeted imaging agents is available. Instrumentation
for the modalities of X-Ray CT, MRI, PET, SPECT and bioluminescence are
available. A cyclotron will be in place within the near future.
- MolMart. This facility provides common molecular
biological reagents and supplies for purchase and delivery to
investigator laboratories. Reagent pick-up at the site is also
available.
- Research
Histology Core. The Research Histology Core offers high quality,
high throughput histology services including routine embedding,
sectioning and H and E and special staining protocols. This core works
in close affiliation with the BTRF to coordinate efforts to support
investigators.
- Tissue Culture Core. Services provide by the Tissue
Culture Core include cell line culture, propagation and storage as well
as primary cell culture and baculovirus production. A variety of cell
culture reagents are available in the laboratory for investigator
purchase.
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