Details -- Canto 2 (V9630067) -- 3 lasers/8 colors.

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Details -- Canto 2 (V9630067) -- 3 lasers/8 colors.

Equipped with an HP xw4600 workstation using Windows XP and FACSDiva software, Version 6.1.2 (build 2008.09.23.06.01).  Firmware 1.47, CST 1.1.2, PLA 1.1.2.

The Canto II  has BD’s QC protocol (CST – Cytometer Setup and Tracking) run on it daily to verify CVs and trends in performance. It is kept under BD service contract; so it receives preventative maintenance (PM) every 6 months and is kept to BD’s performance specs.  The machine is cleaned weekly.  This cytometer was manufactured in October 2008 and put into service at the CIC in August 2009.

FSC = photodiode with 488/10 filter

Octagon (Blue Laser, 488nm, solid state, 20mW)

 

 

Filter 1

Filter 2

A

PE-Cy7

735LP

780/60

B

PerCp-Cy5.5

655LP

670LP

C

(no detector)

610LP

---

D

PE

556LP

585/42

E

FITC

502LP

530/30

F

SSC

---

488/10

G

(no detector)

---

---

H

(no detector)

---

---

 

Trigon (Red Laser, 636nm, HeNe, 17mW)

 

 

Filter 1

Filter 2

A

APC-Cy7

735LP

780/60

B

(no detector)

685LP

---

C

APC

---

660/20

 

Trigon (Violet Laser, 405nm, solid state, 30mW)

 

 

Filter 1

Filter 2

A

AmCyan

502LP

510/50

B

Pac Blue

---

450/50

C

(no detector)

---

---

 

 

Maintenance:

The CIC flow facility is run by Shawn “Trip” Gill, a Systems Engineer with 15 years of experience working with BD flow cytometers.

Basic reagents (sheath fluid, shutdown solution, FACSClean solution) are all BD products used within BD guidelines.

 

Further technical specs can be found on the BD website:

http://www.bdbiosciences.com/instruments/facscanto/index.jsp