The Preclinical Tumor Analysis and Imaging (PTAI) Shared Resource is dedicated to facilitating in vivo cancer research. Our services move research projects from the bench or from a patient’s sample into an in vivo animal model context. The PTAI Shared Resource tests therapies involving small molecules, biologics, and/or radiation. We offer guidance and assistance for the design and performance of standardized or customized protocols for subcutaneous and orthotropic xenograft and syngenic mouse models. The PTAI Shared Resource uses ACUC approved protocols to perform preclinical studies, can follow established protocols, or help investigators design custom protocols. New emphasis is placed on the noninvasive detection and measurement of tumor growth and spread using bioluminescence, PET, or MRI. PTAI was formed by merging the pre-existing Molecular Assessment and Preclinical Studies Shared Resource, which was responsible primarily for management and generation of animal models, with the exceptional technical capabilities of the Small Animal Imaging Shared Resource. We believe this reorganization will better integrate relevant imaging technologies with the analyses of animal tumor models. Staff members have considerable experience and serve as consultants and trainers as well as service providers. They also act as agents to coordinate services in other shared resources, providing a single point of contact for a PI to carry out an entire study that might include culture of cells, immunohistochemistry, flow cytometry, molecular analyses of tumors, and statistics. PTAI provides laboratory services to clinicians, who may not have appropriate laboratory facilities or staff, and initiates animal studies for laboratory scientists who have inadequate experience with in vivo models and otherwise depend solely on in vitro models.