The Center is a
fully equipped, stand-alone biomedical research facility that occupies
the 7th (top) floor of the MultiStory Building in the West
|UVA West Complex (front entrance)
University of Virginia Medical Center on Jefferson Park Avenue in
Charlottesville. This building previously served as the University
Hospital and was entirely renovated in the early 1990s. The corridors
and laboratories are appointed with oak woodwork furniture and
cabinets, using blue as the accent color. Large windows in the
laboratories that run across the front of the building afford views
overlooking of the Medical Center and hills in the nearby
countryside, including Monticello, home of Thomas Jefferson.
The laboratories are connected together thereby
facilitating shared use of equipment, and promoting interactions
between various laboratory groups. On the interior of the floor plan
are faculty offices, desk areas and equipment and tissue culture rooms.
A wide public corridor provides access to the Center administrative
offices, conference room, cold room, and shared equipment and
instruments. Other laboratories on the rear side of the building
have the same arrangement of labs along the outside
walls, providing plenty of natural light to all the laboratories.
The Center offers an outstanding working environment.
Center has all the facilities and instrumentation to culture a variety
of cells and to produce and purify many different natural or
recombinant DNA, proteins and enzymes. The Center for Cell Signaling is
well equipped for research involving biochemistry, molecular biology
and cell biology. Incubators and sterile transfer hoods in multiple
locations allow for the culture of mammalian cells or bacteria or
yeast. Multiple PCR machines are available, including real-time q-PCR.
A dishwashing and autoclave facility supports these activities.
Harvesting of cells and initial fractionation employs a variety of
superspeed and ultracentrifuges. Protein and enzyme purifications may
use one of the multiple FPLC, HPLC, and traditional chromatography
systems in two walk-in coldrooms plus reach-in cold cabinets. Gel
electrophoresis and immunoblotting are available in every lab, and we
have an Odyssey 2D fluorescent scanner for quantification of Westerns.
Stained gels can be analyzed with a CCD camera imaging system, and
those containing radiolabeled molecules with a PhosphorImager
(Molecular Dynamics). Assays can produce colored or fluorescent
products that are analyzed by a UV-visible spectrophotometer or
microtiter plates readers with selectable filters, or radiolabeled
products that are analyzed with a liquid scintillation counter
(Beckman). There are three separate microscope systems for imaging
cells, a Nikon upright microscope for fixed specimens and a Zeiss and a
Nikon inverted microscopes for live specimens. These all have
high-speed digital cameras and OpenLab software study the behavior of normal and cancer
shares these facilities with investigators in the Department of
Radiation Oncology located on the same floor on the North wing of the
building, forming a community of about 50 individual researchers. The
main building is connected by two inside walkways to Jordan Hall, the
home of other basic science departments and research core
Wang, Senior Scientist at work in laboratory.