Targeting Services

Targeting Services

Gene Targeting Services available:

The Gene Targeting and Transgenic Facility can begin a new project by obtaining the genomic clone of the investigator's gene, characterizing it, and constructing the targeting construct.

Accession number or clone

Alternatively, the GTTF can consult with investigators in the design of the targeting construct, and can begin with the investigator's targeting construct.
Following targeting construct synthesis and validation, the GTTF will prepare ES cells and feeder cell dishes, electroporate the cells with the investigator's targeting construct, apply selection conditions to the cultures, pick, expand and freeze approximately 200 clones, isolate DNA samples from duplicate clone cultures, and analyze for targeting using a PCR and Southern blot strategy.

Once appropriately targeted ES cell clones have been identified, they will be expanded, tested by karyotyping and for mycoplasma, and the TMCF will schedule blastocyst injections with the desired number of clones in consultation with the investigator.