GTTF forms
GTTF Forms and Fees for All Services
Fees were approved by the institution on Oct. 22, 2012. UVa Cancer Center members should contact CC for competing funds allocated to support for the use of CC shared resources. Non-UVa users are encouraged to contact us for terms and fees.
|
SERVICE |
TERMS |
FEE |
FORM | |
|
Microinjection |
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| Transgenics-B6SJL F1 | per construct with 3-founder guarantee | $4,490 | online | |
| Transgenics-B6/other strains | per construct with 3-founder guarantee | $7,592 | ibid | |
| Blastocyst injection-129 ESC | per clone with >50 blastocyst injected | $4,888 | online | |
| Blastocyst injection-B6 ESC | per clone with >50 blastocyst injected | $5,562 | ibid | |
|
Gene Targeting |
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| ES cell transfection | 300 drug resistant clones provided for screening | $6,516 | online | |
| Targeted clone recovery | 6 clones recovered and expanded | $1,645 | online | |
| Rosa26 knock-in | 10 PCR positive clones provided | $6,516 | online | |
| ESC karyotyping | per clone with 40 spreads counted | $784 | online | |
| ESC expansion | 4 vials with 2 million cells each | $479 | online | |
|
pMEF derivation iMEF (MEF immortalization) |
3 million cells/per vial (20 vials minimum per order) per project (10 vials provided) |
$69 $2,888 |
online | |
| ESC line derivation | 5 clones under P5, karyotyped and sexed | $7,155 | online | |
| ESC for targeting or DNA prep | 1 vial for targeting/5 vials for DNA prep | $75 | online | |
| Mutation identification | design PCR/Southern assay | $526 | online | |
|
Cryopreservation |
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| Embryo cryo-300 | 300 embryos with test thaw | $3,224 | online | |
| Embryo cryo-200 | 200 embryos with test thaw | $2,407 | ibid | |
| Embryo cryo-100 |
100 embryos with test thaw |
$1,356 | ibid | |
| Sperm cryo | 10 straws of frozen sperm from 2 males | $651 | online |
|
| Embryo implantation | Up to 3 ICR recipient female mice | $515 | online | |
| Embryo isolation | preimplantation/postimplantation embryo isolation | $409 | online | |
| IVF | 200 eggs + sperm from 1 male or 1 vial | $1,226 | online | |
|
Genetic Analysis |
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| Speed congenics-10A | screen 57 markers of 10 mice | $3,286 | online | |
| Speed congenics-10B | screen 28 markers of 10 mice | $1,314 | ibid | |
| Speed congenics-10C | screen 14 markers of 10 mice | $657 | ibid | |
| Speed congenics-10D | screen 7 markers of 10 mice | $329 | ibid | |
| Strain background | screen 57 loci for 5 mice | $1,643 | online | |
| Single locus genotyping | per genotype per mouse | $5 | online | |
| Tail DNA prep | per sample | $8 | ibid | |
| Congenic breeding | per project for 15 months | $5,213 | online | |
| Conventional breeding | 12 cages per month | $282 | ibid | |
|
Transgenic Workshop |
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| Training workshop | 5 days of lectures and hands-on training | $989 | online |
ESC: embryonic stem cell; IVF: in vitro fertilization; MEF: mouse embryonic fibroblast
