The UVa Transgenic Methods and Applications Workshop

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The UVa Transgenic Methods and Applications Workshop

The UVa Gene Targeting and Transgenic Facility holds a hands-on five day intensive workshop during spring (Feb/Mar), summer (Jun/Jul) and fall (Oct/Nov) three times a year.  This workshop includes lectures and hands-on laboratory exercises which is intended for those who wish to develop a strong background in transgenic technologies and applications.   On-line registration is available all year around; please indicate your preferred month on the registration form.  The cost for UVa participants is $980.  UVa faculty and staff may use their education benefits to pay for the workshop.  Please visit HR-benefits website  http://www.hr.virginia.edu/hr-for-you/university-staff/university-staff-benefits/education-benefits and fill an enrollment form  http://www.hr.virginia.edu/forms/all-forms/education-benefits-forms to request for education benefits. Outside participants are encouraged to contact the facility.  Each participant receives a binder containing all materials presented in the workshop along with laboratory protocols and references.  Certificates will be issued to participants who complete the entire course.  The course covers the following areas.

Transgenic technology overview
Early mouse embryo development 
Web search tools for transgenic and knockout mice 
BAC and targeted transgenesis (Rosa26 knock-in)
Making transgenic mice by zygote injection
Preparation of females for embryo collection
Pronuclear microinjection
Generation of pseudopregnant females
Implantation in foster mothers
Identification of transgenic progeny
Homologous recombination in ES cells
Knockout and knock-in construct design
Culture and transfection of ES cells
Generation of gene targeted ES cell clones
Blastocyst microinjection
Optimization of chimeric mouse breeding and germline transmission
Genetic background and speed congenics
Cryopreservation and rederivation
Assisted reproductive technology
Live and video demonstrations
Hands-on exercises (e.g. superovulation; embryo
harvest, transfer and cryopreservation; microinjection).