Traditional development of a congenic strain, which involves serial backcrossing to transfer a gene (allele) from a donor strain to a recipient inbred strain, requires 2.5 to 3 years to complete 10 generations of backcrossing (N10). In contrast, speed congenic strain development relies on microsatellite marker difference (simple sequence length polymorphisms, SSLPs) between strains by selective breeding of progeny with higher percentage of recipient genome, thereby shortening the generations of backcrossing to 5 (N5, 1.25 to 1.5 years). In addition, microsatellite marker-assisted approach can assist linkage analysis and strain background check.
GTTF has taken over the services provided by the previous Mouse Genetics Core. We'll perform genome scanning with a panel of mapped SSLP makers for (1) introgressing target loci onto selected inbred backgrounds; (2) linkage analysis and (3) characterizing genetic backgrounds. Please fill out the relevant request forms in the submenu for the services.