Lymphocyte Culture Center resource description
The Lymphocyte Culture Center (LCC) has been a research support facility of the University of Virginia School of Medicine since January 1983. The primary function of the LCC is to make available to researchers the most current technology and expertise for the construction and selection of lymphocyte-myeloma hybridomas for the production of monoclonal antibodies. The LCC can also provide expertise in the use of these reagents in basic research programs. Services are customized to the specific requirements of individual investigators to optimize the recovery of appropriate antigen specific monoclonal antibodies. The LCC has also provided research support services to other academic institutions and companies, and to federal agencies such as the NIH, the FBI, and USAMRIID.
The center occupies an approximately 1270 square foot modern laboratory equipped with cell culture incubators, laminar flow hoods, centrifuges, inverted and standard microscopes, automated microplate washer and ELISA plate readers, automated HPLC systems, etc. We also have facilities for the cryopreservation and long-term storage of established hybridomas and myeloma cell lines in liquid nitrogen cell banks. The LCC is currently staffed by myself and three laboratory specialists advanced.
The LCC will immunize animals, collect sera and develop ELISA assay strategies appropriate for screening large numbers of hybridoma culture supernatants and animal sera. Once an appropriate assay system has been developed (either in the LCC or in the individual researcher's laboratory) to demonstrate the successful immunization of the necessary animals, fusions are scheduled on a first come first serve basis. The facility conducts all aspects of cell fusion, assaying by ELISA for specific antibody positive cultures, culturing, cloning, freezing, and recovery of specific antibody producing clones. Investigators receive, upon request, hybridoma culture supernatants and the individual hybridoma clones. The center also provides other extended services including bulk expansion of clones, isotyping and sub-isotyping of monoclonal antibodies, bulk monoclonal antibody production in vitro using single use bioreactors, and monoclonal and polyclonal antibody purification by affinity chromatography on recombinant Protein G columns. The LCC also serves as a repository, production facility and distribution point for intellectual property (hybridomas and monoclonal antibodies) licensed by the University of Virginia Patent Foundation.