Flow Cytometry Facility resource description
The Flow Cytometry Core Facility provides all investigators at the University of Virginia access to high quality, cost effective flow cytometry services. In addition to providing access to a variety of instrumentation, the FCCF also provides scientific expertise necessary to effectively use this technology to enhance the scope and quality of scientific research performed at the University.
The state of the art facility occupies approximately 1800 sq. ft. and houses a variety from basic to high end flow cytometry instrumentation. This includes a three laser 8 color FACSVantage SE TurboSort DIVA, a six laser 18 color Reflection Cell Sorters, both which provide 4-way high-speed cell sorting and complex analytical services. In addition, the facility has two 2-laser 4-color FACSCalibur benchtop analyzers as well as a 3-laser 5-color and a 4-laser 10-color analyzers. Other instruments available include a three laser 9-color CyAn ADP LX benchtop cytometer, a four laser LSRFortessa with 16 fluorescent detectors, an ImageStreamX imaging flow cytometer and a Luminex 100 IS bead-based multiplex analyzer. For those requiring flow cytometry access in a BSL-3 environment, the FCCF has expanded services to the BSL3 suite in MR6 with a five laser 17 color high speed Influx cell sorter. A multi-TB server is available for data storage as well as site licenses for data analysis software.
Researchers have the option, once trained, of performing their own analysis or utilizing the expertise of the facility's staff to run their samples for them. Specialized training classes are offered for those researchers who wish to better understand the principles and techniques employed in this technology and prefer to directly acquire and/or analyze their own samples.