DNA Sciences resource description

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DNA Sciences resource description

The DNA Sciences Core Facility provides a variety of services focusing in nucleic acid research. The main purpose is to give UVa investigators access to shared instrumentation and expertise in DNA related disciplines in order to maximize their research capability in a cost effective and time saving manner.

 

Currently the core provides deep sequencing technology in both high throughput and low throughput modes. The Illumina Genome Analyzer IIx provides 15-30 giga bps each run in an 8-lane configuration while the MiSeq 2 provides 2-5 giga bps each run in a single lane format.  The core has operated an Affymetrix GeneChip System for more than 10 years and will continue to provide microarray analysis services to researchers in need of gene expression, microRNA, copy number and SNP analyses using this technology. The core also has provided services in real time PCR instrumentation and experimentation in a scope embracing gene expression, microRNA, copy number and SNP analysis. In addition, the core has been operating Sanger DNA sequencing in an Applied Biosystems 3730 DNA Analyzer to provide services in single reaction, whole-plate run, and targeting amplicon sequencing and BAC and PAC sequencing. Primer walking is also a part of the Sanger sequencing for those project in order to streamline the operation for higher efficiency.

 

Consultation and training in specific techniques and instrumentation is available and are being offered periodically. The DNA Sciences Core has worked closely with other cores in assisting investigators in experimental design, sample preparation, and data analysis. Those include the Biorepository  and Tissue Research Core, the Bioinformatics Core, and the Gene Targeting and Transgenic Facility.