The Advanced Microscopy Facility is pleased to announce two major instrumentation developments:
(1) Zeiss LSM 710 Multiphoton System In Spring 2012, institutional funding facilitated the purchase of a Zeiss LSM 710 Multiphoton-Confocal system. The system, on a Zeiss Axio Examiner upright platform, features: three-channel spectral detection (6 laser lines -405, 458, 488, 514, 561 and 633) for single-photon imaging; a Coherent Chameleon Vision-S laser for 2-photon imaging; external BiG non-descanned detectors; a motorized xy stage; objectives for multiple applications, including dipping lenses for live-cell and intravital imaging; and Zen 2011 software. The system came into service in June 2012.
(2) Zeiss Sigma VP HD field emission SEM Notice was received in May 2012 of funding awarded by the NIH Shared Instrumentation Grant program for acquisition of a field-emission scanning electron microscope to be housed in the Advanced Microscopy Facility. Dr. Jeffrey Corwin, a member of the Advisory Committee for the facility, is the principal investigator on the grant, which was the cooperative effort of those investigators party to the application (Drs. Jeffrey Corwin, Xiaowei Lu, Jung-Bum Shin, Brant Isakson, Edward Botchwey, Alison Criss, James Casanova, James Browne, Stephen Caldwell, and Joanna Goldberg), and Dr. Stacey Guillot, Assistant Director of the AMF. Acquisition of a modern, field emission SEM will provide biomedical researchers access to long-needed instrumentation that can provide significantly improved resolution and the capacity for effective operation at low accelerating voltages (compared to the 1990 vintage SEM currently housed in the AMF). The instrument started operation August 2013.