Handling Recommendations

Handling Recommendations

 

binding

Recommendations for handling purified antibodies

This preparation should be dialyzed against Phosphate Buffered Saline (PBS) or similar isotonic solution commonly used for storing antibodies.  The preparation is currently in a combination of Acetic Acid and Tris at a pH of approximately 7.0.

The activity of the prep should be assayed prior to storage

Solutions of purified antibodies should be stored at relatively high concentrations  (> 1mg/ml) at a neutral pH.  Concentrations up to 10mg/ml are commonly used.  Antibodies at lower concentrations should be concentrated prior to freezing.

Dispense the antibody in convenient volumes and store at -20°C.