Recommendations for handling purified antibodies

This preparation should be dialyzed against Phosphate Buffered Saline (PBS) or similar isotonic solution commonly used for storing antibodies.  The preparation is currently in a combination of Acetic Acid and Tris at a pH of approximately 7.0.

The activity of the prep should be assayed prior to storage

Solutions of purified antibodies should be stored at relatively high concentrations  (> 1mg/ml) at a neutral pH.  Concentrations up to 10mg/ml are commonly used.  Antibodies at lower concentrations should be concentrated prior to freezing.

Dispense the antibody in convenient volumes and store at -20°C.