List of Equipment in Research Cores

Major equipment in research cores

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Equipment	Uses	Core	Use type	Building	Room
JEOL 1230	transmission electron microscope, real-time digital imaging (2Kx2K), ultra high resolution digital imaging (4Kx4K), film imaging.	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
JEOL 100CXII	transmission electron microscope, real-time digital imaging (2Kx2K) and film imaging	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
JEOL 6400	scanning electron microscope with digital image capture and Orion image processing	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Zeiss LSM 510	confocal microscope, inverted, with 4 channels	Advanced Microscopy Facility	core or user operated	MR4	5136
Zeiss LSM 510 with purple diode laser	confocal microscope, inverted, with 4 channels, excitation of DAPI	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Zeiss LSM 510 META/FCS	confocal microscope, inverted, with 3 channels and a programmable detector	Advanced Microscopy Facility	core or user operated	West Complex	Center for Cell Signaling
Zeiss LSM 700	confocal microscope, inverted, with 4 channels, ZEN software	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Zeiss LSM 710	Zeiss LSM 710 Multiphoton confocal. upright, 4 channels, 6 laser lines, Coherent Chameleon IR laser, stage incubator.	Advanced Microscopy Facility	core or user operated	core or user operated
Leica Ultracut UC7 ultramicrotome	preparation of samples for transmission electron microscopy	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Olympus BX51 with digital camera	Brightfield, Fluorescence, Phase Contrast and DIC	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Olympus SZX12 dual objective stereo microscope with digital camera	Brightfield, Darkfield and Fluorescence	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
High Resolution Vacuum Evaporator for rotary shadowing	metal coating	Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Leica Ultracut Ultramicrotomes (3)		Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Critical Point Dryer		Advanced Microscopy Facility	core or user operated	Old Medical School	4806
Sputter Coater		Advanced Microscopy Facility	core or user operated	Old Medical School	4806

Computing clusters, dedicated access, 8 cores, 128GB RAM		Bioinformatics Core
Computing cluster, shared access, over 1500 cores, over 3TB RAM		Bioinformatics Core
Ingenuity Pathway Analysis, Oncomine Premium subscription based bioinformatics software		Bioinformatics Core
Bioconductor software for bioinformatics and statistical computing, over 600 packages		Bioinformatics Core

Bruker Avance III NMR spectrometer 800MHz		Biomolecular Magnetic Resonance Facility	core or user operated	Chemistry	133
Bruker Avance III NMR spectrometer 600MHz		Biomolecular Magnetic Resonance Facility	core or user operated	Chemistry	133
Bruker Avance DRX NMR spectrometer 600MHz		Biomolecular Magnetic Resonance Facility	core or user operated	Chemistry	106
Varian NMRS spectrometer 600MHz		Biomolecular Magnetic Resonance Facility	core or user operated	Chemistry	111
Varian Inova 500 NMR spectrometer 500MHz		Biomolecular Magnetic Resonance Facility	core or user operated	Chemistry	175
Leica ASLMD laser microdissection apparatus	micro scale dissection of fixed or frozen tissue	Biorepository and Tissue Research Facility	user operated	MR6	G712
Arcturus PixCell IIe laser microdissection apparatus	micro scale dissection of fixed or frozen tissue	Biorepository and Tissue Research Facility	user operated	MR6	G712
Arcturus AutoPix laser microdissection apparatus	micro scale dissection of fixed or frozen tissue	Biorepository and Tissue Research Facility	user operated	MR6	B501
Bio-Rad Calligrapher MiniArrayer	production of reverse phase protein arrays	Biorepository and Tissue Research Facility	core operated	MR6	G712
VigeneTech MicroVigene protein microarray analysis software	analysis of reverse phase protein arrays	Biorepository and Tissue Research Facility	core operated	MR6	G710
Pathology Devices TMArrayer semi-automated tissue microarray apparatus	production of tissue microarrays	Biorepository and Tissue Research Facility	core operated	MR6	G710
QIAGEN QIAcube robotic workstation	fully automated protein and nucleic acid extraction	Biorepository and Tissue Research Facility	core operated	MR6	G712
Promega Maxwell16 robotic workstation	fully automated protein and nucleic acid extraction	Biorepository and Tissue Research Facility	core operated	MR6	G712
QIAGEN TissueLyser II	tissue disruption/homogenization for fresh, frozen or fixed tissue	Biorepository and Tissue Research Facility	core operated	MR6	G712
Next Advance Bullet Blender tissue homogenizer	tissue disruption/homogenization for fresh, frozen or fixed tissue	Biorepository and Tissue Research Facility	core operated	MR6	G712
Miltenyi Biotech gentleMACS™ Dissociator	fresh tissue dissociation for viable cells	Biorepository and Tissue Research Facility	core operated	MR6	G712
Bio-Rad iMark Absorbance Microplate Reader	96-well format plate reader; available filters 415,450,595,620,655,750 nm	Biorepository and Tissue Research Facility	core or user operated	MR6	G712
Bio-Rad Experion microcapillary electrophoresis system	microscale/LabChip RNA and DNA analysis	Biorepository and Tissue Research Facility	core operated	MR6	G712
NanoDrop spectrophotometer	microscale DNA and RNA quantitation	Biorepository and Tissue Research Facility	core or user operated	MR6	G712
DAKO Autostainer/Autostainer Plus	automated immunohistochemistry platform	Biorepository and Tissue Research Facility	core operated	MR6	G712
DAKO PT Link pretreatment module	semi-automated depariffinization and antigen retrieval for immunohistochemistry	Biorepository and Tissue Research Facility	core operated	MR6	G712
Leica 3050S research cryostat	preparation of frozen tissue sections	Biorepository and Tissue Research Facility	core or user operated	MR6	G710
Olympus BX41TF with 12 megapixel Digital Camera and Olympus Microsuite Pathology	bright-field photomicroscopy and remote collaboration	Biorepository and Tissue Research Facility	core or user operated	MR6	G712
Nikon COOLSCOPE	bright-field photomicroscopy	Biorepository and Tissue Research Facility	core or user operated	MR6	B520
Leica SCN400 digital whole-slide scanner with SlidePath Digital Image Hub, Tissue IA, and OpTMA Module	bright-field whole slide imaging, image analysis, and tissue microarray de-arraying, annotation, and analysis	Biorepository and Tissue Research Facility	core operated	MR6	B703
Sakura Finetek VIP5 Processor	automated processing of formalin-fixed tissue	Biorepository and Tissue Research Facility	core operated	MR6	G710
Sakura Finetek Tissue-Tek TEC	preparation of paraffin blocks	Biorepository and Tissue Research Facility	core operated	MR6	G710
Microm HM 325 with collimator	microtome for sectioning paraffin blocks and tissue microarrays	Biorepository and Tissue Research Facility	core operated	MR6	G710
MVE-Chart 1536P vapor phase LN2 freezers	vapor phase biospecimen storage	Biorepository and Tissue Research Facility	core operated	MR6	B501
-30º C freezer	biospecimen storage	Biorepository and Tissue Research Facility	core operated	MR6	G701A
-80º C freezers	biospecimen storage	Biorepository and Tissue Research Facility	core operated	MR6	G701A

Applied Biosystems 3730 DNA analyzer	Sanger sequencing of DNA; SNPlex®; genotyping	DNA Sciences	core operated	Jordan	1044
Illumina genome analyzer	analysis of whole chromosomes, targeted genomic regions, BACS, whole genomes, gene expression, small RNA discovery.	DNA Sciences	core operated	Jordan	1044
Illumina MiSeq sequencing system	small, rapid next gen sequencing projects	DNA Sciences	core operated	Jordan	1044
Applied Biosystems Prism 7900HT sequence Detection System	real time PCR	DNA Sciences	core operated	Jordan	1076
Affymetrix Gene Chip System	studying changes in gene expression	DNA Sciences	core operated	Jordan	1071
Agilent Bioanalyzer	capillary electrophoresis of small samples of DNA and RNA for quality control	DNA Sciences	core operated	Jordan	1076
NanoVue Spectrophotometer	measuring absorbances of small volumes, especially quantitation of DNA	DNA Sciences	core operated	Jordan	1076
QIAcube DNA/RNA purification apparatus	nucleic acid extraction	DNA Sciences	core operated	Jordan	1076

SensorMedics Vmax Metabolic Stress Testing Systems	Clinical measurement of oxygen consumption during exercise	Exercise Physiology	Core	West Complex, Private Clinics	5527
BOD POD GS Body Composition tracking system (COSMED)	Air displacement plethysmography system for determining body composition, e.g. fat free tissue and fat tissue	Exercise Physiology	Core	West Complex, Private Clinics	5527
GE Case Exercise Testing System including ECG and Treadmill	Clinical measurement of oxygen consumption during exercise	Exercise Physiology	Core	West Complex, Private Clinics	5527
Monark Arm Crank Ergometer	Upper body ergometer	Exercise Physiology	Core	West Complex, Private Clinics	5527
SensorMedics Deltatrac Bedside Metabolic System	Measurement of basal or resting metabolism	Exercise Physiology	Core	West Complex, Private Clinics (mobile use)	5527
Quinton Q-Stress ECG system	Electrocardiograph for exercise testing; compatible with Lode ergometer	Exercise Physiology	Core	West Complex, Private Clinics	5527
Lode Bicycle Ergometer	Electronically calibrated research ergometer	Exercise Physiology	Core	West Complex, Private Clinics	5527
XCT 2000 Peripheral Quantitative Computed Tomography (pQCT)	Measurement of bone density, geometrical parameters of bone and soft tissue distribution	Exercise Physiology	Core or User	West Complex, Private Clinics	5527
Specialty Software
sliceOmatic (TomoVision)	Medical image analysis software for measurement, segmentation and analyzes of multi-slice scanner data.	Exercise Physiology	Core	West Complex, Private Clinics	5526
Brachial Analyzer (MIA Vascular Research Tools 5)	Medical image analysis software for the assessment of FMD/endothelial function of brachial artery from ultrasound image data.	Exercise Physiology	Core	West Complex, Private Clinics	5526

Becton Dickinson FACSVantage SE Turbo Sorter with DIVA option	4 way sorting, sterile sorting, BSL2 materials, with 3 lasers	Flow Cytometry Facility	core operated	Jordan	2013
Becton Dickinson Fortessa LSRII benchtop analyzer.	4 lasers, 16 color analyses at up to 30,000 cells/sec	Flow Cytometry Facility	core or user operated	Jordan	2011
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer	5 color analyses	Flow Cytometry Facility	core or user operated	Jordan	2011
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer	10 color analyses	Flow Cytometry Facility	core or user operated	Jordan	2011
Becton Dickinson FACSCalibur Benchtop Analyzer	4 color analyses with Automated Micro-Sampler system for 96 well plates	Flow Cytometry Facility	core or user operated	Jordan	2011
Becton Dickinson FACSCalibur Benchtop Analyzer	4 color analyses	Flow Cytometry Facility	user operated	MR4	6123
Beckman Coulter CyAn ADP LX 9 Color	9 color analysis at up to 30,000 cells/sec	Flow Cytometry Facility	core or user operated	Jordan	2011
iCyt Reflection Cell Sorter 12 color	4 way sorting, 12 color detection, BSL2 materials.	Flow Cytometry Facility	core operated	Jordan	1213
Amnis ImagestreamX	imaging cytometer, for dark field, bright field and fluorescent images with 4 lasers, 12 channels, 20X, 40X, 60X magnifications, 96 well plate automation	Flow Cytometry Facility	core or user operated	Jordan	2011
Luminex® 100™ System	measures multiple analytes using multiple assay formats	Flow Cytometry Facility	core or user operated	Jordan	2011
Luminex MAGPIX®	measures multiple analytes such as chemokines from mouse or human sample	Flow Cytometry Facility	core or user operated	Jordan	2011
BD Influx cell sorter for BSL3 samples in custom Baker biosafety cabinet	sorting of BSL3 samples using 5 lasers, 15 colors	Flow Cytometry Facility	core operated	MR6	B414
Data analysis work station (5)	4 Mac stations and 1 PC with FloJo and Modfit software	Flow Cytometry Facility	user operated	Jordan	1215, 2011

Leica DM IRB inverted microscopes		Gene Targeting and Transgenic Facility	core operated	MR5	G339
Leitz micromanipulators		Gene Targeting and Transgenic Facility	core operated	MR5	G339
Sutter P-87 micropipette puller		Gene Targeting and Transgenic Facility	core operated	MR5	G339
De Fonbrune microforge		Gene Targeting and Transgenic Facility	core operated	MR5	G339
Bio-Cool BCIV40A Controlled-Rate Freezer		Gene Targeting and Transgenic Facility	core operated	MR5	G339
XYClone laser system		Gene Targeting and Transgenic Facility	core operated	MR5	G339
BTX ECM630 Electroporator		Gene Targeting and Transgenic Facility	core operated	Jordan	2223
Eppendorf EP Gradient Mastercycler		Gene Targeting and Transgenic Facility	core operated	Jordan	2223
UVIdoc LCD Gel Documentation System		Gene Targeting and Transgenic Facility	core operated	Jordan	2223

Matrix Assisted Laser Desorption Ionization-Time Of Flight mass spectrometer (MALDI-TOF), Bruker Microflex	measure mass of intact molecules, normally peptides, protein, also small pieces of DNA, other molecules	Mass Spectrometry	core or user operated	Jordan	1105
Thermo LTQ Velos Orbitrap mass spectrometer	determination of peptide sequences for protein identification in complex mixtures of proteins, relative quantitation of proteins in mixtures, identification of phosphorylation sites and other post translational modifications	Mass Spectrometry	core operated	Jordan	1105
Thermo TSQ Quantum Access	absolute quantitation of peptides and proteins with appropriate standards. Triple quadrupole machine.	Mass Spectrometry	core operated	Jordan	1105

7 Tesla Bruker/Siemens ClinScan small animal MRI	MRI scans of small animals	Molecular Imaging Core	core or user operated	Snyder Building
4.7 Tesla Varian 200/400 MRI small animal imager with Varian Direct Drive imaging console	MRI scans of small animals	Molecular Imaging Core	core or user operated	MR4
Caliper IVIS Spectrum bioluminescence and fluorescence scanner	fluorescence and luminescence scanner for tissue samples	Molecular Imaging Core	core or user operated	Snyder Building
Siemens Medical Solutions Focus 120 Micro Positron Emission Tomography scanner	scan tissues or small animals using positrons	Molecular Imaging Core		Snyder Building
Siemens Eclipse Cyclotron	production of isotopes for PET scanning	Molecular Imaging Core	core operated (Siemens/PETNET)	Snyder Building
Radiochemistry modules	synthesis of radiolabels	Molecular Imaging Core	core operated	Snyder Building

Tissue processors for formalin and Bouin's fixed tissues		Research Histology Core	core operated	MR4	1123
tissue embedding station		Research Histology Core	core operated	MR4	1123
microtomes	preparing thin sections for microscopy	Research Histology Core	core operated	MR4	1123
stainer, automated	staining of tissue sections	Research Histology Core	core operated	MR4	1123
cryostat	preparing frozen tissue sections for microscopy	Research Histology Core	core operated	MR4	1123


fluorescent plate reader	assays using fluorescent reagents in 6 to 384 well plates	Shared Instrumentation	user operated	Jordan	1074
circular dichroism spectropolarimeter, Aviv 410	secondary structure of proteins, including melting studies	Shared Instrumentation	user operated	Jordan	1074
Bio-Rad FX fluorescent gel scanner	scanning gels stained with Sypro Ruby and other fluorescent stains. 3 laser sources, several filters to cover common visible wavelengths.	Shared Instrumentation	core operated	Jordan	232
Dionex HPLC system for 0.3 to 4.6 mm diameter columns	analysis of small samples of peptides, proteins and other molecules	Shared Instrumentation	core operated	Jordan	1051
Agilent HPLC system for 2 mm diameter columns, UV detector	reverse phase chromatography of small samples, manual fraction collection	Shared Instrumentation	core operated	Jordan	1051
Amersham FPLC	separation of proteins, peptides, and other molecules in aqueous buffers by gel filtration or ion exchange; a steel free system	Shared Instrumentation	core operated	Jordan	232
Bio-Rad GS-800 densitometer	scanning X-ray type films, gels stained with visible stains like Coomassie and silver	Shared Instrumentation	user operated	Jordan	1074
ImageQuant Tl software	relative quantitation of bands on gels and film	Shared Instrumentation	user operated	Jordan	1074
Aperio ScanScope	scanning microscopy slide to digital images at magnifications of 20X, 40X, 63X for digital pathology	Shared Instrumentation	user operated	Jordan	1074
Pharmacia/GE IPGphor isoelectric focusing apparatus, with 18 cm strip holders, Ettan Dalt 6 electrophoresis apparatus	isoelectric focusing	Shared Instrumentation	user operated	Jordan	232
Bio-Rad Protean IEF cell, 11 cm, 17 cm, 24 cm strip holders,Criterion Dodeca cell Protean II Xi cell,Protean Plus Dodeca cell	isoelectric focusing, 2-D gels	Shared Instrumentation	user operated	Jordan	232
Acea xCELLigence RTCA real time cell analysis instrument	real time, continuous measurement of cellular activity, including migration, based on impedance measurements	Shared Instrumentation	user operated	Jordan	1074
Biotek ELx800 absorbance plate reader	absorbance measurements on microtiter plates at 450, 562, 600, 660 nm	Shared Instrumentation	user operated	Jordan	1074

Inverted microscopes, Axio and Olympus		Tissue Culture Facility	core or user operated	Jordan	2-34
Olympus IM microscope		Tissue Culture Facility	core or user operated	Jordan	2-34
CO₂ incubators		Tissue Culture Facility	core or user operated	Jordan	2-34
Incubators	maintenance of insect cell lines	Tissue Culture Facility	core operated	Jordan	2-34
cold storage, -80°C, liquid nitrogen	cell storage	Tissue Culture Facility	core operated	Jordan	2-34
Biosafety cabinets (3)	cell culture	Tissue Culture Facility	user operated	Jordan	2-34

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List of Equipment in Research Cores

List of Equipment in Research Cores

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