Rates
Rates current as of December 2012 are shown.
Payment from outside the University of Virginia can be made with a
credit card, check or wire transfer. See instructions on the web
site of the Office of Research Core Administration
http://www.medicine.virginia.edu/research/Cores/orca/cores-billing-system/instructions/outside_billing-page
Approved grants receive a co-payment from the Cancer Center. The co-payment is automatically applied through the CORES billing system.
Genome Sequencing
| University of Virginia | outside rate | |
| Library preparation, paired end read | $560.00 | $851.20 |
| Library preparation, single end read | $480.00 | $730.00 |
| 42 cycle, paired end read | $1500.00 | $2310.00 |
| 42 cycle, single read | $1080.00 | $1663.00 |
| 84 cycle, paired end read |
$2500.00 | $3850.00 |
| 84 cycle, single read | $1500.00 | $2310.00 |
| MiSeq, 50 cycles | $850.00 | $1292.00 |
| MiSeq, 300 cycles | $1200.00 | $1824.00 |
DNA Sanger Sequencing
|
|
University of Virginia |
Outside rate |
| 1-16 reactions | $7.50 | $11.55 |
| 17-48 reactions | $6.89 |
$10.61 |
| 48+ reactions | $6.63 | $10.21 |
| Full plate sequencing (96 reactions) | $487 |
$749.98 |
Affymetrix GeneChip Expression Analysis
|
University of Virginia |
outside rate |
|
| Total RNA isolation from cells, per sample | $38.00 | $58.52 |
| Total RNA isolation from tissue, per sample | $45.00 | $69.30 |
| RNA quality check by Agilent Bioanalyzer, per well | $9.00 | $13.86 |
| RNA quality check by Agilent Bioanalyzer, per cartridge |
$86.00 | $132.44 |
| full GeneChip analysis service, including data analysis |
$390.00 | $600.60 |
| Affymetrix microarray analysis service without data analysis | $330.00 | $508.20 |
| hybridization and scanning | $65.00 | $100.10 |
| Affymetrix GeneChips; prices vary by chip type | from $250 to $425 | from $250 to $425 |
Real time PCR
|
|
University of Virginia |
Outside rate |
| cDNA synthesis/sample for RT-PCR, 50 µL scale, per RNA 1-16 samples |
$12.00 |
$18.48 |
| cDNA synthesis/sample for RT-PCR, 50 µL scale, per RNA >16 samples |
$10.80 | $17.02 |
| Quantitative real time PCR, 1-48 /gene/sample | $9.00 |
$13.86 |
| Quantitative real time PCR, >16 samples | $8.10 | $12.00 |
| SYBR Green assay design | $128.00 | $197.12 |
Oligonucleotide Synthesis
contact MolMart (email: smd-mol@virginia.edu ) 4-9211 to order through Invitrogen Supply Center
Mass Spectrometry
| University of Virginia | outside University of Virginia | |
| Mass determination by MALDI-staff | $50 | $105 |
| Mass determination by MALDI-Open Access | $30 | N/A |
| High accuracy mass determination by ESI | $250 | $380 |
| Mass determination by electrospray with MS/MS | $300 | $450 |
| Simple Mixture MS/MS | $400 | $690 |
| Peptide Mass Fingerprinting | $250 | $380 |
| de novo sequencing/PTMs | $1,250 | inquire |
| Analysis of Complex mixture | $1,250 | inquire |
| MRM setup (per compound) |
$400 | inquire |
| MRM sample | $100 | inquire |
HPLC, protein interactions, gel scanning
| University of Virginia | outside University of Virginia | |
| HPLC/FPLC setup for one or more samples | $56 | $86.24 |
| HPLC/FPLC per injection, no collection | $46 | 70.84 |
| HPLC/FPLC per injection, collection of fractions | $60 | 92.40 |
| HPLC/FPLC method development | $311 | $471 |
| scan gel (densitometry or fluorescence) | $25 | $61 |
| Scan slide with Aperio ScanScope, standard | $10 | |
| Scan slide, complex or oil immersion lens | $25 |
User operated Instruments
| Service | University of Virginia | outside users |
| Fluorescent plate reader, first 10 minutes | $15 | $23.10 |
| Fluorescent plate reader, per hour after first 10 minutes | $15 | $23.10 |
| Create new account for fluorescent plate reader and introductory training | $28 | $43.12 |
| circular dichroism spectropolarimeter | $28/hr | $43.12/hr |
| Training on Aperio ScanScope | $60 |
