Real Time PCR

Real Time PCR

The core has two instruments, an ABI Prism® 7900 HT Detection System and a Bio-Rad QX200™ Droplet Digital™ PCR System which has enhanced capabilities over other instruments.

 

Applications:

  • quantitation of gene expression
  • absolute quantitation of miRNA
  • mutation and rare sequence detection, 1 in 100,000 with QX200
  • allelic discrimination
  • single nucleotide polymorphisms (SNP) characterization and screening
  • microRNA quantitation
  • DNA copy number variation (CNV), +/- 10% with QX200
  • Pathogen detection and quantitation
  • Single cell analysis detects gene expression differences in a population of cells (QX200)
  • Next Generation Sequencing-digital PCR quantitates samples to increase sequencing accuracy and reduce repeats and enables validation of sequencing results.

For more information about RT-PCR, contact Yongde Bao (434.924.2553, yb8d@virginia.edu)