Shared Instrumentation Services
The Shared Instrumentation Core within the Biomolecular Research Facility has several user and core operated instruments and services, largely for studying proteins.
For other services, contact John Shannon ph. 434.243.9399 to discuss projects aims and sample requirements.
Fluorescent plate reader for reading fluorescent reagents in microtiter plates
Circular dichroism for study of secondary structure of proteins.
Chromatography by HPLC or FPLC of proteins and peptides and other molecules
Gel scanning for visible and fluorescent stains
A spot cutter for cutting spots from gel stained with Sypro Ruby and visible stains
Aperio ScanScope which scans microscope slides for digital pathology
Densitometer for scanning films, blots and gels with visible stains and ImageQuant software for analysis
Isoelectric focusing apparatus for loan for 2-D gels and Bio-Rad Rotofor apparatus.
Absorbance plate reader, with filters for 450, 562, 600, 660 nm
Real time cell analysis instrument
Summary Statement of resources for grant applications.
The Biomolecular Research Facility has discontinued N-terminal sequencing using Edman chemistry;searches at http://www.abrf.org/index.cfm/page/resources/ABRF_Core_Marketplace.htm and elsewhere on the web will find other facilities which perform this analysis.
Protocols
Methanol chloroform precipitation of proteins. This technique has been used to concentrate and desalt proteins.
