Chloroform-Methanol Precipitation of Proteins

Chloroform-Methanol Precipitation of Proteins

A procedure for precipitating proteins from solution, including detergent solutions, is from Wessel and Flügge (1984). To 0.1 ml of solution add 0.4 ml of methanol, mix and centrifuge at 9000g for 10 s; add 0.1 ml of chloroform, mix and spin at 9000g for 10 s. Add 0.3 ml of water, mix vigorously and centrifuge at 9000g for 1 min. Remove upper phase. Add 0.3 ml of methanol to lower phase and interphase with precipitated protein, mix and centrifuge at 9000g for 2 min to pellet protein.

Wessel, D. and Flügge, U.I. (1984) Anal. Biochem. 138 141-143.