Protein Analysis and Identification by Mass Spectrometry

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Protein Analysis and Identification by Mass Spectrometry

The W.M. Keck Biomedical Mass Spectrometry Laboratory within the Biomolecular Research Facility identifies and analyzes proteins and other molecules of biological interest from gels and solution using current mass spectrometry techniques. The laboratory also performs some quantitative analyses

To discuss projects, contact Dr. Nicholas Sherman 434.924.0070.

Analyses available:

  • Protein identification and sequencing from gel or solution by either peptide mass fingerprinting or ESI-LC/MS/MS at the low femtomole level (Simple mixture  MS/MS service).
  • Analysis of protein mixtures such as tissue and media (Complex mixture MS/MS service)
  • protein analysis of samples prepared by Laser Capture Microdissection (GTRF)
  • Proteomics, comparison of proteins in tissue samples using chemical/isotopic labels or label free analysis
  • Absolute quantitation of proteins using labeled peptides and selective reaction monitoring (MRM service)
  • Identification of phosphorylation sites using titanium dioxide enrichment
  • Identification of post-translational modifications (e.g.  ubiquitination) (de novo sequencing service/PTM service)
  • De Novo (manual) sequence analysis of novel proteins to obtain sufficient sequence data for cloning
  • High resolution, high mass accuracy measurements of peptides and small proteins by ESI
  • Mass determination by MALDI mass measurements for confirming identity of peptides and proteins, either by staff or open access self service
  • Identification of interaction partners
  • Quantitative analysis of metabolites, peptides, biomarkers, small molecules by SRM

Sample submission

Good sample preparation is vital in getting good data. When starting a new project, read our guidelines and discuss the project with Dr. Sherman. Enter sample information into LabLink, which is where your data will be posted

Equipment

Statement of resources for grant applications.

Acknowledgements

All investigators are asked to acknowledge the director, Dr. Nicholas E. Sherman and support by the School of Medicine. e.g. "This work used (service and/or instrument name) in the Biomedical Mass Spectrometry Laboratory which is supported by the University of Virginia School of Medicine."

Publications