Products

Products

Media, Cells and Reagents

You can see a list of items on the web at:

https://core.web.virginia.edu/pubview/CurrentItemMstr.cfm?core_nbr=19

In the Search box, click the arrow for the drop down list and select Vendor:TCF then click Go at the end of the line.

Come to room 226 Jordan Hall with PTAO card ( 9 a.m. to 5 p.m. M-F)

  • Find media in cold cabinets, buffers and pipet tips in cabinets against wall nearest to hall.
  • Some items are available by the case-look in book of bar codes near scanner
  • check out items using scanner
  • Bills will be sent the next month.

The items below can only be supplied to researchers at the University of Virginia.

Media

The media below are supplied by Invitrogen

Description Invitrogen Catalog
Number
Unit
Basal Media

DMEM (high glucose) [with 4500 mg/ml D-glucose, L-glutamine, without sodium pyruvate] 11965-092 500 ml
DMEM (low glucose) [with 1000 mg/ml D-glucose, 110 mg/L sodium pyruvate, without L-glutamine, phenol red] 11054-020 500 ml
DMEM (low glucose) [with 1000 mg/ml D-glucose, L- glutamine, 110 mg/L sodium pyruvate] 11885-084 500 ml
DMEM/F-12 (1:1) [with 15 mM HEPES, L-glutamine] 11330-032 500 ml
DMEM/F-12 (1:1) [with L-glutamine, 15 mM HEPES, pyridoxine-HCl, without phenol red] 11039-021 500 ml
DMEM/F-12 (1:1) [with L-glutamine, without HEPES] 11320-033 500 ml
F-12 Nutrient Mixture (Ham's) [with L-glutamine] 11765-054 500 ml
Grace's Insect Medium, supplemented 11605-094 500 ml
IMDM [with L-glutamine, 25 mM HEPES, 3024 mg/L sodium bicarbonate, without α-thioglycerol, β-mercaptoethanol] 12440-046 1000 ml
IMDM [with L-glutamine, 25 mM HEPES, 3024 mg/L sodium bicarbonate, without α-thioglycerol, β-mercaptoethanol] 12440-053 500 ml
McCoy's 5A Medium (modified) [with L-glutamine] 16600-082 500 ml
Medium 199 [with Earle's salts, L-glutamine, 2200 mg/L sodium bicarbonate] 11150-059 500 ml
MEM [with Earle's salts, L-glutamine] 11095-080 500 ml
MEM [with Earle's salts, non-essential amino acids, without L-glutamine] 10370-021 500 ml
Opti-MEM I, reduced serum medium [with HEPES, 2400 mg/L sodium bicarbonate, hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements, growth factors, phenol red reduced to 1.1 mg/L] 31985-070 500 ml
RPMI Medium 1640 [with L-glutamine, without phenol red] 11835-030 500 ml
RPMI Medium 1640 [with L-glutamine] 11875-093 500 ml



Antibiotics & Antimycotics

Antibiotic/Antimycotic (100x) 15240-062 100 ml
Fungizone antimycotic 15290-018 20 ml
Penicillin-Streptomycin (10,000 units/ml) 15140-122 100 ml
Geneticin selective antibiotic (G418 sulfate) 11811-023 1 g
Geneticin selective antibiotic (G418 sulfate) 11811-031 5 g



Balanced Salt Solutions

DPBS (10x) [without calcium, magnesium, phenol red] 14200-075 500 ml
DPBS (1x) [without calcium, magnesium, phenol red] 14190-144 500 ml



Dissociation Reagents

TrypLE™ Express stable trypsin-like enzyme [with phenol red] 12605-010 100 ml
Trypsin-EDTA (1x) [0.05% trypsin, 0.53 mM EDTA-4Na, with phenol red] 25300-054 100 ml
Trypsin-EDTA (10x) [0.5% trypsin, 5.3 mM EDTA-4Na] 15400-054 100 ml



Buffers & Supplements

HEPES Buffer (1M) 15630-080 100 ml
Sodium Bicarbonate (7.5%, w/v) 25080-094 100 ml
B-27 supplement (50x) 17504-044 10 ml
L-Glutamine (200 mM) (100x) 25030-081 100 ml
MEM Non-Essential Amino Acids (10 mM) (100x) 11140-050 100 ml
Sodium Pyruvate (100 mM) (100x) 11360-070 100 ml



Sera

Fetal Bovine Serum, Certified [origin: USA] 16000-044 500 ml
Fetal Bovine Serum, Certified [origin: USA] 16000-xxx 50 ml
aliquot
Fetal Bovine Serum, Qualified [USDA approved sources] 10437-028 500 ml
Horse Serum [origin: New Zealand] 16050-122 500 ml
Newborn Calf Serum [origin: New Zealand] 16010-159 500 ml



Transfection Reagents

Lipofectamine 2000 11668-027 0.75 ml
Oligofectamine 12252-011 1 ml



Competent Cells

MAX Efficiency DH5α competent cells 18258-012 1 ml
Library Efficiency DH5α competent cells 18263-012 1 ml
Subcloning Efficiency DH5α competent cells 18265-017 2 ml
One Shot TOP10 chemically competent E. coli C4040-03 20 rxns
XL1-Blue Subcloning Grade competent cells (Stratagene) 200130 each
XL1-Blue competent cells (Stratagene) 200249 each
XL10-Gold competent cells (Stratagene) 200315 each
BL21-CodonPlus (DE3)-RIL competent cells (Stratagene) 230245 each



PCR Cloning

TOPO TA Cloning Kit (with PCR 2.1-TOPO vector) with One Shot TOP10 E. coli K4500-01 20 rxns