FACSVantage SE DIVA
Becton Dickinson FACSVantage SE Turbo Sorter with DIVA Option
This instrument is capable of high speed and single cell sorting on up to ten parameters (forward & side scatter and 8 fluorescence detectors). The instrument is equipped with three lasers: 488nm air cooled, 633 nm HeNe, and multi-line water cooled laser with UV capability. Pulse Processing capabilities allow the use of area and width signals for doublet discrimination or parameter ratioing for the evaluation of Calcium Flux using Indo-1. The instrument has the capability of four-way sorting into tubes or single cell sorting directly into plates (6-96 wells) or slides. It is equipped with an Aerosol Containment System for the sorting of BSL-2 biohazardous samples. Examples of possible fluorochromes which can be evaluated with this instrument:
- 488nm excitable: FITC, PE, PE-Cy5, PE-Cy5.5, PerCP-Cy5.5, PE-Cy7,
PE-Texas Red, Cy3, GFP, YFP, DsRed, CFSE, Alexa 488, PI, 7-AAD,
Acridine Orange, PKH-26, Dil, DHR123, Fura Red, Fluo-3, DCFDA, SYTOX
Green, DRAQ5, Ethidium Bromide, JC-1, TMR...
- 633nm excitable: APC, APC-Cy5.5, APC-Cy7, Cy5, Alexa 633, Alexa
647, TO-PRO-3, DRAQ5...
- 351nm (UV) excitable: Hoechst 33342, Hoechst 33258, DAPI, Alexa
350, EBFP, Indo-1, Fluorogold...
Consult the facility for available filter set ups, fluorochrome combinations, and appropriate controls, prior to designing your experiment. Located in Jordan Hall, Room 2013; 434-243-2695.
See Scheduling for information on scheduling time on this instrument.