FACSCaliburs
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzers
There are four FACSCalibur instruments in the FlowCore facility:
- FACSCalibur A uses FlowJo Collectors' Edition Acquisition software (Quick Start guide) (Users guide) as well as the Cytek AMS software.
- FACSCalibur B uses CellQuest Pro and Rainbow Acquisition software (Video).
- FACSCalibur C & D use CellQuest Pro Acquisition software.
See the chart below for specific instrument configurations and optical layouts (click on FACSCalibur name):
| Number of Colors |
| FACSCalibur A^ | FACSCalibur B | FACSCalibur C | FACSCalibur D (MR4) |
* A total of two channels can be selected with FACSCalibur B; either 2 off the 407nm or 1 off the 407nm and 1 off the 637nm. See instructions on using the Rainbow Acquisition software.
^ FACSCalibur A can run samples in a 96-well plate with the Automated Micro-Sampler (AMS) system. Download the Cytek AMS software to create layouts on your PC and save time.
Consult the facility for fluorochrome combination feasibility and necessary controls prior to designing your experiment. Feel free to use the FACSCalibur A Staining Panel Worksheet to organize your experiment.
Located in Jordan Hall, Room 2011; 434-243-2711.
See Scheduling for information on scheduling time on these instruments.
