Vascular Lab

Vascular Lab

 

 Vascular Surgery Research Laboratory, University of Virginia

 

About Us:

Our lab was established by Dr. Gilbert R. Upchurch Jr. in the year of 1999 as a part of the Conrad Jobst Vascular Research Laboratories in University of Michigan. In September of 2010, Dr. Upchurch’s Lab moved to Virginia and merged with Dr. Ailawadi’s Lab, forming the Vascular Surgery Research Lab at the University of Virginia. Our present location is on the third flood of MR4 building .The Vascular Research Lab consisted of a molecular biology unit, a histology unit, a microsurgery room, offices and cold storage room. We are affiliated to the Department of Surgery and are part of the Robert M. Berne Cardiovascular Research Center of University of Virginia. It is one of the few labs in the nation that is dedicated solely to the research of aortic aneurysm.

 

Personnel:

                     Principal Investigators                            

            Upchurch

  Gilbert R. Upchurch Jr. M.D.    

 AilawadiG

           Gorav Ailawadi M.D.          

 

 Faculty & Staff

                         Guanyi Lu, M.D., Ph.D. Research Scientist                           

                         Gang Su, M.D., Senior Laboratory Specialist                         

             Yunge Zhao, Ph.D., Research Scientist, Collaborator              

  Ashish K. Sharma, Ph.D., Assistant Professor, Collaborator  

                           Morgan A. Salmon, Ph.D., Research Scientist                          

                                Akshaya Meher, Ph.D., Research Scientist                              

 

  Post-doctoral Research Fellow

                            John P. Davis, M.D., General Surgery Resident                             

                           Nick Pope, M.D.,  General Surgery Resident                             

 

         Student          

                                                                           None

                

 Past members of the Lab:

Jip L. Tolenaar, M.D.,  2013,  Postdoctoral fellow(MD),  Stem cell Rx on ruptured AAA model

William F Johnston, M.D., 2011-2013, Postdoctoral fellow(MD), IL-1β & aromatase in AAA formation

Sean English M.D., 2010-2012, Postdoctoral fellow(MD), Development of ruptured AAA model 

Paul Dimusto, M.D., 2009-1011, Postdoctoral fellow(MD), Serine proteinases in AAA formation

Adriana Laser, M.D., 2009-1011, Postdoctoral fellow(MD), Estrogren receptor in AAA formation

Abhijit Ghosh, Ph.D.,  2009-2010, Research associate,  AKT in AAA formation

Karen J. Roelofs, D.V.M., 2001-2010, Senior Research Specialist, Multiple projects

Omar Sadiq, B.S., 2008-2010,  Student (undergraduate, Role of JNK in AAA formation

Brebdan McEvoy, B.S., 2009-2010,  Student (BS), Gender differences in AAA formation

John Futchko, 2008-2010, Student (undergraduate), Role of ERK in AAA formation

Lauren K. Ehrlichman, M.D., 2008, Predoctoral fellow(BA), Role of ERK in AAA formation

Wagner Tedeschi, M.D.,  2008, Postdoctoral fellow(MD), Gender differences in AAA formation

Kevin K. Hannawa, M.D., 2001-2008, Predoctoral fellow(BA), NO in regulating MMP-9 in SMCs

Nathan Narashimhan, M.D., 2004-2006, Postdoctoral fellow(MD, Role of selectins in AAA formation

Brenda S. Cho, Ph.D., 2003-2006, Predoctoral fellow(MA), Role of gender in AAA formantion

Chris Longo, M.D., 2004-2005, Postdoctoral fellow(MD), CCR2 in AAA formation

Derek T. Woodrum, M.D., 2003-2005, Predoctoral fellow(MD), Role of eNOS in AAA formation

Indranil Sinha , M.D., 2002-2005, Predoctoral fellow(BA), NO prevents MMP-9 mediated AAA

Jonathan L. Eliason, M.D., 2002-2004,  Postdoctoral fellow (MD, Neutrophil depletion on AAA

Vladimir Grigoryants, M.D., 2003-2004, Postdoctoral fellow(MD), Inhibition of AAA by tamoxifen

Gorav Ailawadi, M.D., 2000-2003, Postdoctoral fellow(MD, Differential expression of MMP-9

Brian S. Knipp,M.D., 2001-2003, Predoctoral fellow (MS), Inhibition NOS up-regulates MMP-9

Andrew Lipnick, MD, 2001-2002, Predoctoral fellow(BA), MMPs in DVT resolution

Matthew J. Eagleton, M.D., 2000-2002, Postdoctoral fellow(MD), NO inhibition on MMP expression

David A Peterson, M.D.,  2000-2002, Predoctoral fellow(BA), NF-κB on MMP-expression

Autumn Broady, 2000-2001, Student (undergraduate), Effects of NO on MMP production

 

Research Projects

1. Gender differences in experimental aneurysm formation.

  • Sex hormones in aneurysm formation ( estrogen, testosterone, progesterone)
  • Hormone receptors in aneurysm formation  ( ERα, ERβ, AR)
  • Effect of bone marrow transplant & aortic transplant during aneurysm formation.
  • Inflammatory cytokines & angiogenesis in aneurysm formation.

           

2. Stem cell application in the treatment of aortic aneurysm (mouse, rat & pig model).

3.  Effects of dietary phytoestrogen in aneurysm formation.

4.  Anti-aneurysm drug screening (animal study, biological test).

          

Fundings

       Current fundings: 

  • 2009-2013 NIH (RO1), PI, Gender differences in aortic aneurysm.
  • 2011 - 2013 Astra Zeneca, Pl, Novel systemic targets to inhibit experimental aneurysm formation                                                                                                                             

 

      Completed fundings: 

  • 2007-2012 NIH (RO1), PI, Gender differences in aortic aneurysm.                                           
  • 2001-2006   NIH Mentored Clinical Scientist Development Award (KO8), PI.
  • 2001-2006   Lifeline Foundation® Research Award, PI.

 

  Lab Equipment

  • Real-time PCR CFX96 System—BIO-RAD
  • Gel Doc XR+ Imaging System, Bio-Rad Laboratories, Inc
  •  iMark Microplate Reader, Bio-Rad Laboratories, Inc.
  • Nanodrop 2000 sectrophotometer, ND-2000-US-CAN
  • Incubator, Carbon Dioxide, Thermo Scientific; NAPCO 8000 Series
  • Incubator Shaker II; Boekel; w/3 shelves; 3 trays; sealed metal door w/window
  • Shaker, Rotating; Thermo Scientific; Speed 0 to 25rpm; 3-dimensional, 6deg through 360deg angle rotation; Continuous-duty motor;
  • Trans-Blot SD Semi-dry Transfer Cell, Bio-Rad Laboratories, Inc
  • Ultra-Low Temperature Freezers, Thermo Scientific Revco Plus Series
  • 4 C° Biofuge Fresco, Kendro
  • Centrifuge, 5810R, 4X250 RTR, 15&50ML ADAP;
  • Ultrasonic Cleaners, Branson XCell SureLockᵀᴹ Mini-Cell, Invitrogen
  • Eclipse microscope (Nikon)
  • Surgical microscope (Nikon SMX-800) with NIS-Elements D.3.10 software
  • Microsurgery instruments
  • Ultrasound Pocket Doppler with 4MHz Probe for Vascular Monitoring
  • Multiple computers

 

 Research Techniques

1. Small animal (mouse & rat) microsurgery:

  • Abdominal aortic aneurysm (elastase perfusion model, elastase topical application model, calcium chloride model, angiotensin model, BAPN rupture model).
  • Thoracic aortic aneurysm (topical application model only).
  • Aneurysm induction on bone marrow transplant mouse.
  • Aortic transplant.
  • Heterotopical heart transplant (abdominal model, cervical model, re-transplant, second-graft transplant, target gene transfection).
  • Other “non-micro” survival surgeries (orchiectomy, ovariectomy, drug pellet or device implant etc.)

     

 2. Large animal surgery:

  • Aortic aneurysm induction, organ and tissue transplants, other survival surgeries.

       

3. Biology:

  • Protein, RNA and DNA abstraction.
  • Western blot.
  • RT-PCR.
  • Gelatin zymography & reverse zymography.
  • Cytokine and angiogenesis array.
  • ELISA
  • Elispot
  • Flow cytometry.
  • Cell cultures.
  • Bone marrow transplantation.

     

4. Pathology:

  • Tissue sample processing and slice preparations.
  • Regular histology stainings (HE, Verhoeff van Gieson, Picrosirius red,Trichrome, etc.) 
  • Immunohistochemical stainings (neutraphil, macrophage,CD3⁺ T cell, α-smooth muscle cell actin)

 

5. Others:

  • Mouse colony management & genotyping.
  • Drug dosing (I.V., I.P., I.H, gavage).
  • Sample collecting ( tail vein, retro-orbital, lavage) 

 

 

Publications:

Upchurch Lab Research Archive    (updated 2/17/2014)

 

1.  Aromatase Deletion Increases Female Experimental Aortic Aneurysm Formation William F. Johnston, Morgan Salmon, Gang Su, Guanyi Lu, Emilie F. Rissman, Gorav Ailawadi, Gilbert R. Upchurch Jr. J Vas Surg  (submitted & accepted)

2.  Increased 18F-FDG Uptake in the Aortic Wall of β-Aminopropionitrile Exposed Rats May Be Predictive of AAA Rupture.  English SJ, Piert MR,Diaz JA, Ghosh A, Sherman PS,Quesada CA, Pechota A, Eliason JL,Henke PK, Upchurch GR. Ann Surg  (submitted & accepted)

3.  Dietary phytoestrogens inhibit experimental aneurysm formation in male mice. Lu G, Su G, Zhao Y, Johnston WF, Sherman NE, Rissman EF, Lau C, Ailawadi G, Upchurch GR Jr. J Surg Res. 2013 Dec 3. pii: S0022-4804(13)02140-9. doi: 10.1016/j.jss.2013.11.1108.

4.  Phosphorylation of AKT and Abdominal Aortic Aneurysm Formation.  Ghosh A, Lu G, Su G, McEvoy B, Sadiq O, Dimusto PD, Laser A, Futchko JS, Henke PK, Eliason JL, Upchurch GR Jr. Am J Pathol. 2014 Jan;184 (1):148-58. doi: 10.1016/j.ajpath.2013.09.016.PMID: 24332015 [PubMed - in process] 

5.  Increased estrogen receptor alpha in experimental aortic aneurysms in females compared with males.  Laser A, Ghosh A, Roelofs K, Sadiq O, McEvoy B, Dimusto P, Eliason J, Upchurch GR Jr. J Surg Res. 2013 Aug 18. doi:pii: S0022-4804(13)00723-3. 10.1016/j.jss.2013.07.050. [Epub ahead of print]PMID:23993200  [PubMed - as supplied by publisher] 

6.  Experimental abdominal aortic aneurysm formation is mediated by IL-17 and attenuated by  mesenchymal stem cell treatment.  Sharma AK, Lu G, Jester A, Johnston WF, Zhao Y, Hajzus VA, Saadatzadeh MR, Su G, Bhamidipati    CM, Mehta GS, Kron IL, Laubach VE, Murphy MP, Ailawadi G, Upchurch GR Jr. Circulation. 2012 Sep 11;126(11 Suppl 1):S38-45. Erratum in: Circulation. 2012 Oct 23;126(17):e278. PMID:22965992  [PubMed - indexed for MEDLINE] 

7.  The role of extracellular signal-related kinase during abdominal aortic aneurysm formation.  Ghosh A, DiMusto PD, Ehrlichman LK, Sadiq O, McEvoy B, Futchko JS, Henke PK, Eliason JL, Upchurch GR Jr.  J Am Coll Surg. 2012 Nov;215(5):668-680.e1. doi: 10.1016/j.jamcollsurg.2012.06.414. Epub 2012 Aug 21. PMID:22917644  [PubMed - indexed for MEDLINE] 

8.  Differential gender- and species-specific formation of aneurysms using a novel method of inducing abdominal aortic aneurysms.  Laser A, Lu G, Ghosh A, Roelofs K, McEvoy B, DiMusto P, Bhamidipati CM, Su G, Zhao Y, Lau CL,  Ailawadi G, Eliason JL, Henke PK, Upchurch GR Jr.  J Surg Res. 2012 Dec;178(2):1038-45. doi: 10.1016/j.jss.2012.04.073. Epub 2012 May 18.

9.  Increased JNK in males compared with females in a rodent model of abdominal aortic aneurysm.  DiMusto PD, Lu G, Ghosh A, Roelofs KJ, Sadiq O, McEvoy B, Su G, Laser A, Bhamidipati CM, Ailawadi  G, Henke PK, Eliason JL, Upchurch GR Jr. J Surg Res. 2012 Aug;176(2):687-95. doi: 10.1016/j.jss.2011.11.1024. Epub 2011 Dec 14. PMID:22316675  [PubMed - indexed for MEDLINE]  Free PMC Article

10.  Increased PAI-1 in females compared with males is protective for abdominal aortic aneurysm formation in a rodent model.  DiMusto PD, Lu G, Ghosh A, Roelofs KJ, Su G, Zhao Y, Lau CL, Sadiq O, McEvoy B, Laser A, Diaz JA, Wakefield TW, Henke PK, Eliason JL, Upchurch GR Jr. Am J Physiol Heart Circ Physiol. 2012 Apr 1;302(7):H1378-86. doi: 10.1152/ajpheart.00620.2011. Epub 2012 Feb 3. PMID:22307671  [PubMed - indexed for MEDLINE]  Free PMC Article

11.  Decreased collagen and increased matrix metalloproteinase-13 in experimental abdominal aortic aneurysms in males compared with females.  Cho BS, Roelofs KJ, Ford JW, Henke PK, Upchurch GR Jr. Surgery. 2010 Feb;147(2):258-67. doi: 10.1016/j.surg.2009.06.047. Epub 2009 Sep 20. PMID:19767051   [PubMed - indexed for MEDLINE]  Free PMC Article

12.  Gender-dependent differential phosphorylation in the ERK signaling pathway is associated with increased MMP2 activity in rat aortic smooth muscle cells. Ehrlichman LK, Ford JW, Roelofs KJ, Tedeschi-Filho W, Futchko JS, Ramacciotti E, Eliason JL, Henke PK, Upchurch GR Jr. J Surg Res. 2010 May 1;160(1):18-24. doi: 10.1016/j.jss.2009.03.095. Epub 2009 May 8. PMID:19592018  [PubMed - indexed for MEDLINE]  Free PMC Article

13.  Differential regulation of aortic growth in male and female rodents is associated with AAA development. Cho BS, Woodrum DT, Roelofs KJ, Stanley JC, Henke PK, Upchurch GR Jr. J Surg Res. 2009 Aug;155(2):330-8. doi: 10.1016/j.jss.2008.07.027. Epub 2008 Aug 27.     PMID:19111327  [PubMed - indexed for MEDLINE]  Free PMC Article

14.  Differential effect of 17-beta-estradiol on smooth muscle cell and aortic explant MMP2. Woodrum DT, Ford JW, Cho BS, Hannawa KK, Stanley JC, Henke PK, Upchurch GR Jr. J Surg Res. 2009 Jul;155(1):48-53. doi: 10.1016/j.jss.2008.07.003. Epub 2008 Aug 15. PMID:19041098  [PubMed - indexed for MEDLINE]  Free PMC Article 

15.  Differential regulation of the superoxide dismutase family in experimental aortic aneurysms and rat aortic explants. Sinha I, Pearce CG, Cho BS, Hannawa KK, Roelofs KJ, Stanley JC, Henke PK, Upchurch GR Jr. J Surg Res. 2007 Apr;138(2):156-62. Epub 2006 Dec 29. PMID:17196988 [PubMed - indexed for MEDLINE] 

16.  Female gender attenuates cytokine and chemokine expression and leukocyte recruitment in experimental rodent abdominal aortic aneurysms. Sinha I, Cho BS, Roelofs KJ, Stanley JC, Henke PK, Upchurch GR Jr. Ann N Y Acad Sci. 2006 Nov;1085:367-79. PMID: 17182958 [PubMed - indexed for MEDLINE]

17.  Attenuation of experimental aortic aneurysm formation in P-selectin knockout mice. Hannawa KK, Cho BS, Sinha I, Roelofs KJ, Myers DD, Wakefield TJ, Stanley JC, Henke PK, Upchurch GR Jr. Ann N Y Acad Sci. 2006 Nov;1085:353-9. PMID: 17182955 [PubMed - indexed for MEDLINE] 

18.  Diffusion of Alexa Fluor 488-conjugated dendrimers in rat aortic tissue. Cho BS, Roelofs KJ, Majoros IJ, Baker JR Jr, Stanley JC, Henke PK, Upchurch GR Jr. Ann N Y Acad Sci. 2006 Nov;1085:294-305. PMID: 17182946 [PubMed - indexed for MEDLINE]

19.  Early increased MT1-MMP expression and late MMP-2 and MMP-9 activity during Angiotensin II induced aneurysm formation. Eagleton MJ, Ballard N, Lynch E, Srivastava SD, Upchurch GR Jr, Stanley JC. J Surg Res. 2006 Oct;135(2):345-51. Epub 2006 May 22. PMID: 16716358 [PubMed - indexed for MEDLINE]

20.  A biologic basis for asymmetric growth in descending thoracic aortic aneurysms: a role for matrix metalloproteinase 9 and 2. Sinha I, Bethi S, Cronin P, Williams DM, Roelofs K, Ailawadi G, Henke PK, Eagleton MJ, Deeb GM, Patel HJ, Berguer R, Stanley JC, Upchurch GR Jr. J Vasc Surg. 2006 Feb;43(2):342-8. PMID: 16476613 [PubMed - indexed for MEDLINE]

21.  The nitric oxide donor DETA-NONOate decreases matrix metalloproteinase-9 expression and activity in rat aortic smooth muscle and abdominal aortic explants. Sinha I, Hannawa KK, Ailawadi G, Woodrum DT, Ford JW, Henke PK, Stanley JC, Eagleton MJ, Upchurch GR Jr. Ann Vasc Surg. 2006 Jan;20(1):92-8. PMID: 16378139 [PubMed - indexed for MEDLINE]  Related citations

22.   Alterations in angiotensin converting enzyme during rodent aortic aneurysm formation. Eagleton MJ, Cho B, Lynch E, Roelofs K, Woodrum D, Stanley JC, Upchurch GR Jr. J Surg Res. 2006 May;132(1):69-73. Epub 2005 Dec 2. PMID: 16325859 [PubMed - indexed for MEDLINE]

23.  Mitochondrial-dependent apoptosis in experimental rodent abdominal aortic aneurysms. Sinha I, Sinha-Hikim AP, Hannawa KK, Henke PK, Eagleton MJ, Stanley JC, Upchurch GR Jr. Surgery. 2005 Oct;138(4):806-11. PMID: 16269312 [PubMed - indexed for MEDLINE]

24.  Gender differences in rat aortic smooth muscle cell matrix metalloproteinase-9. Woodrum DT, Ford JW, Ailawadi G, Pearce CG, Sinha I, Eagleton MJ, Henke PK, Stanley JC, Upchurch GR Jr.J Am Coll Surg. 2005 Sep; 201(3):398-404. PMID: 16125073 [PubMed - indexed for MEDLINE]

25.   Neutrophil depletion inhibits experimental abdominal aortic aneurysm formation. Eliason JL, Hannawa KK, Ailawadi G, Sinha I, Ford JW, Deogracias MP, Roelofs KJ, Woodrum DT, Ennis TL, Henke PK, Stanley JC, Thompson RW, Upchurch GR Jr. Circulation. 2005 Jul 12;112(2):232-40. PMID: 16009808 [PubMed - indexed for MEDLINE]  Free Article

26.   L-selectin-mediated neutrophil recruitment in experimental rodent aneurysm formation. Hannawa KK, Eliason JL, Woodrum DT, Pearce CG, Roelofs KJ, Grigoryants V, Eagleton MJ, Henke PK, Wakefield TW, Myers DD, Stanley JC, Upchurch GR Jr. Circulation. 2005 Jul 12;112(2):241-7. Epub 2005 Jul 5. PMID: 15998669 [PubMed - indexed for MEDLINE]  Free Article

27.  Tamoxifen up-regulates catalase production, inhibits vessel wall neutrophil infiltration, and attenuates development of experimental abdominal aortic aneurysms. Grigoryants V, Hannawa KK, Pearce CG, Sinha I, Roelofs KJ, Ailawadi G, Deatrick KB, Woodrum DT, Cho BS, Henke PK, Stanley JC, Eagleton MJ, Upchurch GR. J Vasc Surg. 2005 Jan;41(1):108-14. PMID: 15696052 [PubMed - indexed for MEDLINE] 

28.   Gender differences in experimental aortic aneurysm formation. Ailawadi G, Eliason JL, Roelofs KJ, Sinha I, Hannawa KK, Kaldjian EP, Lu G, Henke PK, Stanley JC, Weiss SJ, Thompson RW, Upchurch GR Jr. Arterioscler Thromb Vasc Biol. 2004 Nov;24(11):2116-22. Epub 2004 Aug 26. PMID: 15331435 [PubMed - indexed for MEDLINE]  Free Article

29.   Increased MMP-9 expression and activity by aortic smooth muscle cells after nitric oxide synthase inhibition is associated with increased nuclear factor-kappaB and activator protein-1 activity. Knipp BS, Ailawadi G, Ford JW, Peterson DA, Eagleton MJ, Roelofs KJ, Hannawa KK, Deogracias MP, Ji B, Logsdon C, Graziano KD, Simeone DM, Thompson RW, Henke PK, Stanley JC, Upchurch GR Jr. J Surg Res. 2004 Jan;116(1):70-80. PMID: 14732351 [PubMed - indexed for MEDLINE] 

30.  Ultrasound measurement of aortic diameters in rodent models of aneurysm disease. Knipp BS, Ailawadi G, Sullivan VV, Roelofs KJ, Henke PK, Stanley JC, Upchurch GR Jr. J Surg Res. 2003 Jun 1;112(1):97-101. PMID: 12873440 [PubMed - indexed for MEDLINE]  PMID: 12844103 [PubMed - indexed for MEDLINE]

31.  A nonintrinsic regional basis for increased infrarenal aortic MMP-9 expression and activity. Ailawadi G, Knipp BS, Lu G, Roelofs KJ, Ford JW, Hannawa KK, Bishop K, Thanaporn P, Henke PK, Stanley JC, Upchurch GR Jr. J Vasc Surg. 2003 May;37(5):1059-66. PMID: 12756355 [PubMed - indexed for MEDLINE] 

32.  Nitric oxide inhibition increases aortic wall matrix metalloproteinase-9 expression. Eagleton MJ, Peterson DA, Sullivan VV, Roelofs KJ, Ford JA, Stanley JC, Upchurch GR Jr. J Surg Res. 2002 May 1;104(1):15-21.

33.   PMID: 11971672 [PubMed - indexed for MEDLINE] Nitric oxide synthase inhibition in vitro is associated with increased nuclear Factor-кB activity in rat aortic smooth muscle. Peterson DA, Wakefield TW, Ford JW, Deogracias MP, Knipp BS, Eagleton MJ, Simeone DM, Stanly JC, Upchurch GR, Jr. Surgical Forum 2001 Vol LII; 370-2

34.   Heparin reacts with and inactivates nitric oxide. Upchurch GR, Jr., Welch GN, Zhang YY, George D, Ye S, Costello K, Loscalzo J. J Cardiovasc Pharm Therapeut. 2001; 6(2):163-73

35.  Upchurch GR, Jr.,Ford JW, Weiss SJ, Knipp BS, Peterson DA, Thompson RW, Eagleton MJ, Broady AJ, Proctor MC, Stanley JC. Nitric oxide inhibition increases MMP-9 expression by rat smooth muscle cells in vitro. J Vasc Surg 2001; 34:76-83

 

Research Training 

1. Resident: Resident research training is an important part of our lab. Our research team has many years of experiences working with those in the resident training programs.  Dr. Upchurch has nurtured many young trainees becoming successful academic surgeons. (links to the related web pages)

2. Post-doctoral fellow and graduate student: Dr. Upchurch is one of the member faculty and mentors of the Cardiovascular Research Center (CVRC) of University of Virginia and also a professor of Molecular Physiology. Our lab supports the Ph.D. training program in cardiovascular biology in the CVRC (link to CRVC web page). Dr. Upchurch could be your thesis advisor. Junior scientist will get excellent research training and experience in vascular biology under the mentorship of Dr. Upchurch (link to CRVC web page). While the lab has all the basic equipments and techniques for aneurysm research, the post-doc and graduate student can also get access to CVRC’s core facilities and shared facilities.

3.  Others: Visiting scholars are welcome to the lab to exchange research ideas. There are also limited opportunities for medical students to receive short term training or to experience scientific research in the field of vascular surgery.

   

             Joining Us?        

 For training and working opportunities in our lab, please contact: 

Gilbert R. Upchurch Jr. MD

 Email: gru6n@virginia.edu

 University of Virginia Health System

Department of Surgery, PO Box 800679

Charlottesville,  VA  22908

 Tel: 434-243-6333,  Fax: 434-243-9941

Lab Contact:

For research collaboration and technical assistant,

please contact our faculty and stuff at the following address:

3153 MR4 Building, 409 Lane Road,

University of Virginia Health System

PO Box 801359

Charlottesville, VA 22908

Tel: 434-924-5329    Fax: 434-924-1218