Other Techniques Used
|Exposure of cells to
hypoxia-reoxygenation. This chamber is used to expose cells to
hypoxia. After flushing the chamber with nitrogen, the pO2 of the
culture media is reduced to 5% within 10 min, and when the valve is
released, the pO2 of the media returns to normoxia (21% O2).
Production of reactive oxygen species (ROS) after hypoxia-reoxygenation. Isolated, primary CD4+ T cells exposed to hypoxia-reoxygenation generate abundant ROS (left), which is attenuated by adenosine A2A receptor agonist (right). Gren fluorescence, from incubation with DCA dye, indicates ROS production.
Flow Cytometry. Use of flow cytometry is used often to identify cell populations within the lung and to identify which cells are producing specific markers or cytokines. Below, flow cytometry is used to demonstrated successful reconstitution of CD4+ T cells into lungs of Rag-1 knockout mice after ischemia-reperfusion (right) vs. Sham (center).
Lung arteriograms. Below is an example of perfusion of rat lungs with a barium sulfate gelatin solution which fills the arterial side of the vasculature (it does not pass through the capillaries). These arteriograms depicts vascular changes in the lung of a rat after sham surgery (Sham), removal of upper two right lobes (R-LBX2), and removal of right lung (R-LBX3).