Molecular & Cell Biology

 

Molecular & Cell Biology

Examples of Molecular & Cell Biology Techiques used in our lab:

Western blot showing upregulation of hepatoma-derived growth factor (HDGF) 7 days after pneumonectomy.  

western blot

Protein array used to scan for angiogenesis-related proteins upregulated in mouse lung after pneumonectomy.

protein array

EMSA showing activation of NFkappaB in pig lung 30 and 60 minutes after transplant.
EMSA

Supershift analysis of NF-kB components demonstrating activation of the p65 subunit.
supershift
Immunohistochemistry detection of infiltration of neutrophils and CD3+ T cells in mouse lungs after ischemia-reperfusion (IR) versus sham.
immunohistochemistry
 H&E-stained pig lung sections demonstrating lung injury and edema after ischemia-reperfusion (B & E) versus sham (A & D)..
lugn histology

Immunohistochemistry of mouse lung showing upregulation of HDGF (hepatoma-derived growth factor) after pneumonectomy.

immunohistochemistry

 

Reactive oxygen species (ROS) generation by DCF dye. Alveolar epithelial cells (top) and macrophages (bottom) exposed to hyoxia-reoxygenation produced marked ROS which is attenuated by apocynin, an NADPH oxidase inhibitor.
ROS production