Animal Models

Animal Models

In our lab, Residents, Postdocs and students utilize relevant animal models of lung injury, lung preservation, transplantation, growth and injury. These models include:
  • Lung transplant in pigs
  • Ex Vivo Lung Perfusion (EVLP)
  • Mouse in vivo hilar clamp model of lung ischemia-reperfusion
  • Isolated, buffer-perfused mouse lung system
  • Pneumonectomy (mice & rats) and lobectomy (rats and swine)
  • Cardiopulmonary bypass in rats
  • Trachea transplant in mice

Isolated mouse lungs. We use an isolated mouse lung system to measure pulmonary function in mouse lungs. This is a buffer-perfused system which is why the lungs are white in the movie below.

Mouse left hilar clamp. We utilize an in vivo mouse model of lung ischemia-reperfusion injury via left hilar ligation.

 

Mouse in vivo lung model

Porcine EVLP. Ex vivo lung perfusion (EVLP) of non-heart beating donor (NHBD) porcine lungs.
PigEVLP2

Mouse left hilar clamp. Shown here is a clamp placed around the left hilum, which is comprised of the left pulmonary artery and left bronchus. When clamped (or lilgated), the left lung is rendered ischemic until the clamp (or ligation) is released.
Hilar clamp

Pig lung transplantation. We perform porcine lung transplantation as a very clinically relevant model for human lung transplantation.
Pig lung transplant2

Mouse pneumonectomy. Compensatory growth of the right lung after left pneumonectomy (right) in mice compared to normal left+right lungs (left). The time point shown is two weeks after pneumonectomy.

cell_PNXmouse.jpg