TCV Surgery Techniques

TCV Surgery Techniques

Our lab uses many surgical, cellular, and molecular techniques including:

Cell/Molecular Techniques:

Western blot
ELISA & Bio-Rad's multiplex assay
Multiplex, multi-cytokine assay
Immunohistochemistry
Cell culture
Real-time PCR
Affymetrix gene-chip array
Northern/Southern blot
EMSA
Ribonuclease Protection Assay (RPA)        
Oxidative stress assays
Phase-contrast & fluorescent microscopy
Primary isolation and culture of
pulmonary immune and epithelial cells

Surgical/Other Techniques:

Transgenic/knockout mouse studies
Murine lung function
Ex vivo lung perfusion
Lung histology & morphometry
Lung bronchoalveolar lavage (BAL)
Rodent lung barium arteriogram and CT imaging
Bone marrow transplant in mice
Adoptive transfer of T cells into mice
Immune cell ablation in mice
Flow cytometry of lung and cultured cells