Our lab uses many surgical, cellular, and molecular techniques
including:
| Cell/Molecular
Techniques:
Western blot
ELISA & Bio-Rad's multiplex assay
Multiplex, multi-cytokine assay
Immunohistochemistry
Cell culture
Real-time PCR
Affymetrix gene-chip array
Northern/Southern blot
EMSA
Ribonuclease Protection Assay
(RPA)
Oxidative stress assays
|
Surgical/Other
Techniques:
Transgenic/knockout mouse studies
Lung histology & morphometry
Lung bronchoalveolar lavage (BAL)
Rodent lung barium arteriogram and CT imaging
Bone marrow transplant in mice
Adoptive transfer of T cells into mice
Immune cell ablation in mice
Primary isolation and culture of various
pulmonary immune and epithelial
cells
Phase-contrast & fluorescent microscopy
Flow cytometry of lung and cultured cells
|
