Scott Vande Pol, M.D., Ph.D.
Scott Vande Pol, M.D.,
Associate Professor of
||M.D., University of California, San
||Ph.D., University of California, San
||Resident in Pathology, National Cancer
Institute, National Institutes of Health, Bethesda, MD 20892
||Postdoctoral Fellow, Laboratory of Tumor
Virus Biology, National Cancer Institutes, Bethesda, MD 20892
Our studies of the BPV-1 E6 oncoprotein found that it associated
with a cellular regulatory adapter molecule called paxillin. We
showed that paxillin was required for the tyrosine phosphorylation of
Focal Adhesion Kinase, a central regulator of integrin signaling, cell
attachment and migration. We now have evidence that paxillin is
required for the activity of many cancer-associated oncoproteins. We
are elucidating the role of paxillin in cancer signal transduction and
A second project has been devoted to understanding of the mechanism by
which E6 oncoproteins activate the ubiquitin mediated degradation of
tumor supressor proteins, such as p53. We are engaged in a
collaboration to solve the solution structure of the E6. In
the process of these studies, we identified interaction motifs on E6
that allow for the interaction of E6 with tumor
supressors. We have recently identified a new
cellular protein that is targeted for degradation by E6 together with a
cellular ubiquitin ligase. This new target is a tyrosine
phosphatase about which little is known. We suspect that by
targeting this phosphatase for degradation, that E6 manipulates
cellular signaling to facilitate the viral life cycle. Given that
human HPV containing tumors all express E6, this phosphatase may prove
to be an important target in the development of cancers, both viral
positive and negative.
- Wade, R., and S. Vande Pol. 2006. Minimal features
of paxillin that are required for the tyrosine phosphorylation of focal
adhesion kinase. Biochem J 393:565-73.
- Cooper, B., Brimer, N., Stoler, M., and Vande Pol,
S.B. 2006. Suprabasal Overexpression of Beta-1
Integrin is induced by Bovine Papillomavirus Type 1. Virology .
2006 Nov 10;355(1):102-14. Epub 2006 Aug 8. PMID: 16899269.
- Brimer, N., Lyons, C., and Vande Pol, S.B.
2006. Association of E6AP (UBE3A) with Human Papillomavirus Type
11 E6 Protein. Virology. 2007 Feb 20;358(2):303-10. Epub 2006 Oct
4. PMID: 17023019
- Jing, M., Bohl, J., Brimer, N., Kinter, M., and
Vande Pol, S.B. 2006. Degradation of
Tyrosine Phosphatase PTPN3 (PTPH1) by association with Oncogenic Human
Papillomavirus E6 Proteins J Virol. 2007 Mar;81(5):2231-9.
Epub 2006 Dec 13. PMID: 17166906
- Bohl, J., Brimer, N., Lyons, C., and Vande Pol,
S.B. 2007. The stardust family protein MPP7 forms a
tripartite complex with LIN7 and DLG1 that regulates the stability and
localization of DLG1 to cell junctions. J Biol Chem. 2007 Mar
30;282(13):9392-400. Epub 2007 Jan 19. PMID: 17237226
- Cooper, B., Brimer, N., and Vande Pol, S.B. Human
Papillomavirus E6 Regulates the Cytoskeleton Dynamics of Keratinocytes
Through Targeted Degradation of p53. J Virol. 2007 Nov;81(22):12675-9.
Epub 2007 Sep 5. PMID: 17804489
- Wade, R., Brimer, N., and Vande Pol, S.B.
Transformation by Bovine Papillomavirus Type 1 E6 Requires
Paxillin. J. Virol. 2008 Jun;82(12):5962-6. Epub 2008 Apr 2.PMID: