Hui Li, Ph.D.

Hui Li, Ph.D.

Hui Li, Ph.D. Faculty Profile

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Hui Li, Ph.D.

Assistant Professor



Education and Training:

1998, B.S. Chemical Physics, University of Science and Technology of China, China

2003, Ph.D. Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, OH

2003-2006, Postdoctoral Associate, Pathology, Yale University, New Haven, CT

2006-2009 Associate Research Scientist, Pathology, Yale University, New Haven, CT


Research:

Gene fusion is a common feature of cancer. The fusion products were believed to be unique to cancer cells until recently we demonstrated that abundant level of fusion products can also be present in normal cells. In these normal cells, the fusion products are made by a post-transcriptional process called “trans-splicing” to serve important functions. Our long-term hypotheses are that such trans-splicings are more than rare events and they play critical roles in normal physiology. In addition, mis-regulated trans-splicing machinery could generate highly abundant fusion products, which would promote oncogenesis the same way as the products made by traditional chromosomal rearrangements.  To discover more RNA trans-splicing events, we propose two approaches, a candidate gene approach and a genome-wide approach.  The candidate gene approach would focus on the known gene fusions associated with chromosomal translocations in various tumors.  We predict that at least some of the fusion products can also be detected in the normal corresponding cells at certain conditions. The key is “when” and “where” to look for such events. 

For the genome-wide approach, we propose a modified “mate-pair” combined with  “paired-end” deep sequencing approach to document the whole transcriptome trans-splicing events in the cells of interest. To validate the candidates of trans-splicing, we are developing an intra-cellular based trans-splicing assay with high throughput potential. Our preliminary studies support the second part of the hypothesis that at least some fusions are the mis-regulated products of trans-splicing. Gain of function and loss of function approaches will be used to study the effect of the trans-spliced chimeric products on cancer development. Our proposed trans-splicing study will enhance our knowledge of the genetic information flow, suggest a novel oncogenic pathway, and provide additional links between cancer and its normal origin cells. In addition, the proposed study of trans-splicing will have both immediate and long-term clinical implications.

Selected Publications:

  • Hui Li*, Jinglan Wang, Xianyong Ma and Jeffrey Sklar (2009) Gene fusions and RNA Trans-splicing in Normal and Neoplastic Human Cells. Cell Cycle 2009 January 8:2, 1-5(* corresponding author)
  • Hui Li, Jinglan Wang, Gil Mor and Jeffrey Sklar (2008) A Neoplastic Gene Fusion Mimics Trans-splicing of RNAs in Normal Cells. Science 2008 September 5; 321,1357-1361.
  • Hui Li, XianYong Ma, Jinglan Wang, Jason Koontz, Marisa Nucci and Jeffrey Sklar (2007) Effects of rearrangement and allelic exclusion of JJAZ1/SUZ12 on cell proliferation and survival. Proc Natl Acad Sci U S A. 2007 December 11; 104(50): 20001-20006.
  • Hui Li, Lois Myeroff, Dominic Smiraglia, Michael F. Romero, Theresa P. Pretlow, Lakshmi Kasturi, Jim Lutterbaugh, Ronald M. Rerko, Graham Casey, Jean-Pierre Issa, Joseph Willis, James K.V. Willson, Christoph Plass and Sanford Markowitz (2003) SLC5A8: A Novel Sodium Transporter, is a Tumor Suppressor Gene Silenced by Methylation in Human Colon Aberrant Crypt Foci and Cancers. Proc Natl Acad Sci U S A. 2003 Jul 8; 100(14): 8412-7.
  • Hui Li, Lois Myeroff, Lakshmi Kasturi, Lisa Krumroy, Stuart Schwartz, James KV Willson, Eric Stanbridge, Graham Casey and Sanford Markowitz (2002) Chromosomal autonomy of hMLH1 methylation in colon cancer. Oncogene 21, 1443-1449.
  • Hui Li and Qing-xiang Guo. (1998) Recent Progress in Studies on the Application of Cationic Liposome to Gene Therapy. Ziran Zazhi (Chinese Nature) 20(5): 275-8.

 

Timeline for Dr. Li's Lab:

     7/2009             Dr. Li started the lab.

     7/2009             Hong Park joined the lab and assisted in setting it up.

     9/2009             Anna Lee joined the lab as an undergraduate volunteer.

     9/2009             Dr. Gong Mei joined the lab as a Research Associate.

   10/2009             Dr. Huiling Yuan joined the lab as a Research Associate.

     3/2010             Dr. Li was awarded American Cancer Society IRG.

     5/2010             Dr. Li was awarded Funds for Excellence in Science and Technology.

     5/2010             Merritt Tuttle joined the lab as an undergraduate volunteer.

     5/2010             Jongoh Lim joined the lab as a summer undergraduate volunteer.

     9/2010             Marina Piper joined the lab as an undergraduate volunteer.

   10/2010             Dr. Li was awarded V Scholarship.

   12/2010             Dr. Yanmei Zhang joined the lab as a Research Associate.

   01/2011             Callie Horn joined the lab as an undergraduate volunteer.

   01/2011             Salma Nabi joined the lab as an undergraduate volunteer.

   04/2011             Dr. Li was awarded Stand Up To Cancer Innovative Research Grant.