Real Time PCR
Consultation: Steven Cohn, M.D., Ph.D., MB/GE Core
Consultation: Alda Vidrich, Ph.D.
Technician: Chibuzo Ilo
Location: MR4 Building, room 1001
Hours of Operation: 9am to 5pm
This Core service provides Digestive Health Research Center members consultation and training in the design of primers for real time PCR, training in the use of the SDS7000 and the Biorad iCycler. Center members will have the option of running real time PCR assays themselves or having them run by our experienced staff. We offer the option of performing SYBR Green or TaqMan real time PCR assays. The reagents needed for these assays are on site and are available to center members at a substantial discount. Additionally, Celera pre-calibrated assays for many genes of interest are available through the core at a discount to Center members. The core also provides assay validation for both SYBR Green and Taqman assays. Finally, The MB/GE core staff provides assistance in data analysis and interpretation and troubleshooting any assay problems.