Articles & Publications
We describe a novel mechanism for vital fluorescent dye entry into sensory cells and neurons: permeation through ion channels. In addition to the slow conventional uptake of styryl dyes by endocytosis, small styryl dyes such as FM1-43 rapidly and specifically label hair cells in the inner ear by entering through open mechanotransduction channels.
A scanning electron micrograph of the surface of a bullfrog’s saccule that shows a hair bundle and cuticular plate that have separated from the soma of a gentamicin-damaged hair cell. Exposure to short-term, low-dose gentamicin causes loss of the sensory apparatus from hair cells, which can survive and recover. Electron microscopy, time-lapse, and multi-photon recordings were used to observe bundle separation, survival of bundleless hair cells, and repair of damaged hair cell surfaces as described in Gale et. al.
|The Journal of Neuroscience, Volume 21,
Number 2, January 15, 2001
Glial growth factor (GGF2) strongly stimulates S-phase entry in the early postnatal rat utricle. DIC micrograph of a piece of rat utricular sensory epithelium composed only of supporting and hair cells, and cultured for 72 hr in a medium containing GGF2. Nuclei of cells that entered S-phase have incorporated BrdU (red). Fluorescent DAPI staining revealed the nuclei that did not enter S-phase in the same piece of epithelium (green). For details, see the article by Montcouquiol and Corwin in this issue (pages 570-580).
|JARO Volume 1, Number 2, September
Extramacular hair cell from the bullfrog saccule, labeled with HCS1,
a specific hair cell antibody, in red. Green is phalloidin
label. For a detailed description, see the paper by Gales,
Meyers, and Corwin in this issue.
Selected Recent Publications
- Corwin, J. and Cotanche, D. 1988. Regeneration of sensory hair cells after acoustic trauma. Science, 240: 1772-1774.
- Forge, A., Li, L., Corwin, J., and Nevill, G. (1993) Ultrastructural evidence for hair cell regeneration in the mammalian inner ear. Science 259:1616-1619.
- Warchol, M., Lambert, P., Goldstein, B., Forge, A., and Corwin, J. (1993) Regenerative proliferation in inner ear sensory epithelia from adult guinea pigs and humans. Science 259:1619-1622.
- Kelley, M., Xu, X., Wagner, M., Warchol, M., and Corwin, J. (1993) The developing organ of cortin contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture. Development 119:1041-1053.
- Warchol, M. and Corwin, J. (1993) Supporting cells in avian vestibular organs proliferate in serum-free culture. Hear Res. 71:28-36.
- Kelley, M., Talreja, D. and Corwin, J. (1995) Replacement of haircells after laser microbeam irradiation in cultured organs of Corti from embryonic neonatal mice. J. Neurosci. 15:3013-3026.
- Warchol, M. and Corwin, J. (1996) Regenerative proliferation in organ cultures of the avian cochlea: Identification of the initial progenitors and determination of the latency of the proliferative response. J. Neurosci. 16: 5466-5477.
- Jones, J. and Corwin, J. (1996) Regeneration of sensory cells after laser ablation in the lateral line system: Hair cell lineage and macrophage behavior revealed by time-lapse video microscopy. J. Neurosci. 16: 649-662
- Saffer, L., Gu, R. and Corwin, J. (1996) An RT-PCR analysis of mRNA for growth factor receptors in damaged and control sensory epithelia of rat utricles. Hearing Res. 94: 14-23.
- Corwin, J., Warchol, M., Saffer, L., Finley, J., Gu, R., and Lambert, P. (1996) Growth factors as potential drugs for the sensory epithelia of the ear. In: Growth factors as drugs for neurological and sensory disorders. Ciba Foundation Symposium 1996. John Wiley and Sons, Chichester (pp. 167-187).
- Warchol, M.E. and Corwin, J.T. (1996) Regenerative
proliferation in organ cultures of the avian cochlea: Identification of
the initial progenitors and determination of the latency of the
J. Neurosci. 16: 5466-5477.
- Kil, J. Warchol, M.E. and Corwin, J.T. (1997) Cell death, cell proliferation, and estimates of hair cell life spans in the vestibular organs of chicks. Hearing Res. 114: 117-126
- Corwin, J.T. (1997) (Guest Editor of this volume) Development of the Ear. Seminars in Cell and Developmental Biology, Academic Press 8: 215-216.
- Oberholtzer, J. Carl and Corwin, J.T. (1997) Fish 'n Chicks: Model Recipes for Hair Cell Regeneration? Neuron (invited minireview) 19: 951-954.
- Gale, J., Meyers, J. R., and Corwin, J. T. 2000. Solitary hair cells are distributed throughout the Extramacular Epithelium in the Bullfrog's Saccule. J.A.R.O. 1:172-182.
- Montcouquiol, M. and Corwin, J. T. 2001a. Intracellular Signals that control Cell Proliferation in Mammalian Balance Epithelia: Key Roles for PI 3-kinase, mTOR, and S6 Kinases in preference to Calcium, PKC, and MAPK. J. Neurosci. 21:570-580.
- Montcouquiol, M. and Corwin, J. T. 2001b. Brief treatments
with forskolin enhance S-phase entry in balance epithelia from
J. Neurosci. 21:974-982.
- Witte, M., Montcouquiol, M., and Corwin, J. T. 2001. Regeneration in avian hair cell epithelia: Identification of Intracellular Signals Required for S-phase Entry. Eur. J. Neurosci. 14:829-838.
- Gale, J. E., Meyers, J. R., Periasamy, A., and Corwin, J. T. 2002. Survival of bundle-less hair cells and subsequent bundle replacement in the bullfrog saccule. J. Neurobiol. 50:81-92.
- Meyers, J.R., MacDonald, R.B., Duggan, A., Lenzi, D., Corwin, J.T., and Corey, D.P. 2003. Lighting up the senses: FM1-43 loading of sensory cells through non-selective ion channels. J Neurosci. 23: 4054-4065.