List of Equipment in Research Cores

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List of Equipment in Research Cores

Major equipment in research cores

 

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Equipment

Uses

Core

Use type

Building

Room

JEOL 1230

transmission electron microscope, real-time digital imaging (2Kx2K),  ultra high resolution digital imaging (4Kx4K), film imaging.

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

JEOL 6400

scanning electron microscope with digital image capture and Orion image processing

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Zeiss LSM 510

confocal microscope, inverted, with 4 channels

Advanced Microscopy Facility

core or user operated

MR4

5136

Zeiss LSM 510 META/FCS

confocal microscope, inverted, with 3 channels and a programmable detector

Advanced Microscopy Facility

core or user operated

West Complex

Center for Cell Signaling

Zeiss LSM 700

confocal microscope, inverted, with 4 channels, ZEN software

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Zeiss LSM 710

Zeiss LSM 710 Multiphoton confocal. upright, 4 channels, 6 laser lines, Coherent Chameleon IR laser,

Advanced Microscopy Facility

core or user operated

core or user operated

 

Leica Ultracut UC7 ultramicrotome

preparation of samples for transmission electron microscopy

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Olympus BX51 with digital camera

Brightfield, Fluorescence, Phase Contrast and DIC

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Olympus SZX12 dual objective stereo microscope with digital camera

Brightfield, Darkfield and Fluorescence

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

High Resolution Vacuum Evaporator for rotary shadowing

metal coating

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Leica Ultracut Ultramicrotomes (3)

 

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Critical Point Dryer

 

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Sputter Coater

 

Advanced Microscopy Facility

core or user operated

Old Medical School

4806

Computing clusters, dedicated access, 8 cores, 128GB RAM

Bioinformatics Core

Computing cluster, shared access, over 1500 cores, over 3TB RAM

Bioinformatics Core

Ingenuity Pathway Analysis, Oncomine Premium subscription based bioinformatics software

Bioinformatics Core

Bioconductor software for bioinformatics and statistical computing, over 600 packages

Bioinformatics Core

 

 

 

 

 

 

Bruker Avance III NMR spectrometer 800MHz

 

Biomolecular Magnetic Resonance Facility

core or user operated

Chemistry

133

Bruker Avance III NMR spectrometer 600MHz

 

Biomolecular Magnetic Resonance Facility

core or user operated

Chemistry

133

Bruker Avance DRX NMR spectrometer 600MHz

 

Biomolecular Magnetic Resonance Facility

core or user operated

Chemistry

106

Varian NMRS  spectrometer 600MHz

 

Biomolecular Magnetic Resonance Facility

core or user operated

Chemistry

111

Varian Inova 500 NMR spectrometer 500MHz

 

Biomolecular Magnetic Resonance Facility

core or user operated

Chemistry

175

Leica ASLMD laser microdissection apparatus

micro scale dissection of fixed or frozen tissue

Biorepository and Tissue Research Facility

user operated

MR6

G712

 

Arcturus (Life Technologies) XT-TI laser microdissection apparatus

 

micro scale dissection of fixed or frozen tissue under bright-field, phase contrast or fluorescence visualization



Biorepository and Tissue Research Facility

user operated

MR6

G712

Pathology Devices TMArrayer semi-automated tissue microarray apparatus

production of tissue microarrays

Biorepository and Tissue Research Facility

core operated

MR6

G710

QIAGEN QIAcube robotic workstation

fully automated protein and nucleic acid extraction

Biorepository and Tissue Research Facility

core operated

MR6

G712

Promega Maxwell16 robotic workstation

fully automated protein and nucleic acid extraction

Biorepository and Tissue Research Facility

core operated

MR6

G712

QIAGEN TissueLyser II

tissue disruption/homogenization for fresh, frozen or fixed tissue

Biorepository and Tissue Research Facility

core operated

MR6

G712

Next Advance Bullet Blender tissue homogenizer

tissue disruption/homogenization for fresh, frozen or fixed tissue

Biorepository and Tissue Research Facility

core operated

MR6

G712

Miltenyi Biotech gentleMACS™ Dissociator

fresh tissue dissociation for viable cells

Biorepository and Tissue Research Facility

core operated

MR6

G712

Bio-Rad iMark Absorbance Microplate Reader

96-well format plate reader; available filters 415,450,595,620,655,750 nm

Biorepository and Tissue Research Facility

core or user operated

MR6

G712

Bio-Rad Experion microcapillary electrophoresis system

microscale/LabChip RNA and DNA analysis

Biorepository and Tissue Research Facility

core operated

MR6

G712

NanoDrop spectrophotometer

microscale DNA and RNA quantitation

Biorepository and Tissue Research Facility

core or user operated

MR6

G712

DAKO Autostainer/Autostainer Plus

automated immunohistochemistry platform

Biorepository and Tissue Research Facility

core operated

MR6

G712

DAKO PT Link pretreatment module

semi-automated depariffinization and antigen retrieval for immunohistochemistry

Biorepository and Tissue Research Facility

core operated

MR6

G712

Leica 3050S research cryostat

preparation of frozen tissue sections

Biorepository and Tissue Research Facility

core or user operated

MR6

G710

Olympus BX41TF with 12 megapixel Digital Camera and Olympus Microsuite Pathology

bright-field photomicroscopy and remote collaboration

Biorepository and Tissue Research Facility

core or user operated

MR6

G712

Nikon COOLSCOPE

bright-field photomicroscopy

Biorepository and Tissue Research Facility

core or user operated

MR6

B520

Leica SCN400 digital whole-slide scanner with SlidePath Digital Image Hub, Tissue IA, and OpTMA Module

bright-field whole slide imaging, image analysis, and tissue microarray de-arraying, annotation, and analysis

Biorepository and Tissue Research Facility

core operated

MR6

B703

Sakura Finetek VIP5 Processor

automated processing of formalin-fixed tissue

Biorepository and Tissue Research Facility

core operated

MR6

G710

Sakura Finetek Tissue-Tek TEC

preparation of paraffin blocks

Biorepository and Tissue Research Facility

core operated

MR6

G710

Microm HM 325 with collimator

microtome for sectioning paraffin blocks and tissue microarrays

Biorepository and Tissue Research Facility

core operated

MR6

G710

MVE-Chart 1536P vapor phase LN2 freezers

vapor phase biospecimen storage

Biorepository and Tissue Research Facility

core operated

MR6

B501

-30º C freezer

biospecimen storage

Biorepository and Tissue Research Facility

core operated

MR6

G701A

-80º C freezers

biospecimen storage

Biorepository and Tissue Research Facility

core operated

MR6

G701A

 

 

 

 

 

 

Applied Biosystems 3730 DNA analyzer

Sanger sequencing of DNA; SNPlex®; genotyping

DNA Sciences

core operated

Jordan

1044

Illumina genome analyzer

analysis of whole chromosomes, targeted genomic regions, BACS, whole genomes, gene expression, small RNA discovery.

DNA Sciences

core operated

Jordan

1044

Illumina MiSeq sequencing system

small, rapid next gen sequencing projects

DNA Sciences

core operated

Jordan

1044

Applied Biosystems Prism 7900HT sequence Detection System

real time PCR

DNA Sciences

core operated

Jordan

1076

Affymetrix Gene Chip System

studying changes in gene expression

DNA Sciences

core operated

Jordan

1071

Agilent Bioanalyzer

capillary electrophoresis of small samples of DNA and RNA for quality control

DNA Sciences

core operated

Jordan

1076

NanoVue Spectrophotometer

measuring absorbances of small volumes, especially quantitation of DNA

DNA Sciences

core operated

Jordan

1076

QIAcube DNA/RNA purification apparatus

nucleic acid extraction

DNA Sciences

core operated

Jordan

1076

 

 

 

 

 

 

SensorMedics Vmax Metabolic Stress Testing Systems

Clinical measurement of oxygen consumption during exercise

Exercise Physiology

Core

Barringer Wing

5527

BOD POD GS Body Composition tracking system (COSMED)

Air displacement plethysmography system for determining body composition, e.g. fat free tissue and fat tissue

Exercise Physiology

Core

Barringer Wing

5527

GE Case Exercise Testing System including ECG and Treadmill

Clinical measurement of oxygen consumption during exercise

Exercise Physiology

Core

Barringer Wing

5527

Monark Arm Crank Ergometer

Upper body ergometer

Exercise Physiology

Core

Barringer Wing

5527

SensorMedics Deltatrac Bedside Metabolic System

Measurement of basal or resting metabolism

Exercise Physiology

Core

Barringer Wing (mobile use)

5527

Quinton Q-Stress ECG system

Electrocardiograph for exercise testing; compatible with Lode ergometer

Exercise Physiology

Core

Barringer Wing

5527

Lode Bicycle Ergometer

Electronically calibrated research ergometer

Exercise Physiology

Core

Barringer Wing

5527

XCT 2000 Peripheral Quantitative Computed Tomography (pQCT)

Measurement of bone density, geometrical parameters of bone and soft tissue distribution

Exercise Physiology

Core or User

Barringer Wing

5527

Specialty Software

 

 

 

 

 

sliceOmatic (TomoVision)

Medical image analysis software for measurement, segmentation and analyzes of multi-slice scanner data.

Exercise Physiology

Core

Barringer Wing

2406

Brachial Analyzer (MIA Vascular Research Tools 5)

Medical image analysis software for the  assessment of FMD/endothelial function of brachial artery  from ultrasound image data.

Exercise Physiology

Core

Barringer Wing

2406

 

 

 

 

 

 

Becton Dickinson FACSVantage SE Turbo Sorter with DIVA option

4 way sorting,  sterile sorting,  BSL2 materials, with 3 lasers

Flow Cytometry Facility

core operated

Jordan

2013

Becton Dickinson Fortessa LSRII benchtop analyzer.

4 lasers, 16 color analyses at up to 30,000 cells/sec

Flow Cytometry Facility

core or user operated

Jordan

2011

Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer

5 color analyses

Flow Cytometry Facility

core or user operated

Jordan

2011

Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer

10 color analyses

Flow Cytometry Facility

core or user operated

Jordan

2011

Becton Dickinson FACSCalibur Benchtop Analyzer

4 color analyses with Automated Micro-Sampler system for 96 well plates

Flow Cytometry Facility

core or user operated

Jordan

2011

Becton Dickinson FACSCalibur Benchtop Analyzer

4 color analyses

Flow Cytometry Facility

user operated

MR4

6123

Beckman Coulter CyAn ADP LX 9 Color

9 color analysis at up to 30,000 cells/sec

Flow Cytometry Facility

core or user operated

Jordan

2011

iCyt Reflection Cell Sorter 12 color

4 way sorting, 12 color detection, BSL2 materials.

Flow Cytometry Facility

core operated

Jordan

1213

Amnis ImagestreamX Mark II

imaging cytometer, for dark field, bright field and fluorescent images with 4 lasers, 12 channels, 20X, 40X, 60X magnifications, 96 well plate automation

Flow Cytometry Facility

core or user operated

Jordan

2011

Luminex® 100™  System

measures multiple analytes using multiple assay formats

Flow Cytometry Facility

core or user operated

Jordan

2011

Luminex MAGPIX®

measures multiple analytes such as chemokines from mouse or human sample

Flow Cytometry Facility

core or user operated

Jordan

2011

BD Influx cell sorter for BSL2 samples in custom Baker biosafety cabinet

sorting of BSL2 samples using 5 lasers, 15 colors

Flow Cytometry Facility

core operated

Jordan

2013

DVS Sciences CyTOF 2 analysis of multiple parameters at single cell level Flow Cytometry Facility core operated Jordan 2013

Data analysis work station (5)

4 Mac stations and 1 PC with FloJo and Modfit software

Flow Cytometry Facility

user operated

Jordan

1215, 2011

 

 

 

 

 

 

Leica DM IRB inverted microscopes

 

Gene Targeting and Transgenic Facility

core operated

MR5

G339

Leitz micromanipulators

 

Gene Targeting and Transgenic Facility

core operated

MR5

G339

Sutter P-87 micropipette puller

 

Gene Targeting and Transgenic Facility

core operated

MR5

G339

De Fonbrune microforge

 

Gene Targeting and Transgenic Facility

core operated

MR5

G339

Bio-Cool BCIV40A Controlled-Rate Freezer

 

Gene Targeting and Transgenic Facility

core operated

MR5

G339

XYClone laser system

 

Gene Targeting and Transgenic Facility

core operated

MR5

G339

BTX ECM630 Electroporator

 

Gene Targeting and Transgenic Facility

core operated

Jordan

2223

Eppendorf EP Gradient Mastercycler

 

Gene Targeting and Transgenic Facility

core operated

Jordan

2223

UVIdoc LCD Gel Documentation System

 

Gene Targeting and Transgenic Facility

core operated

Jordan

2223

 

 

 

 

 

 

Matrix Assisted Laser Desorption Ionization-Time Of Flight mass spectrometer (MALDI-TOF), Bruker Microflex

measure mass of intact molecules, normally peptides, protein, also small pieces of DNA, other molecules

Mass Spectrometry

core or user operated

Jordan

1105

Thermo LTQ Velos Orbitrap mass spectrometer

determination of peptide sequences for protein identification in complex mixtures of proteins, relative quantitation of proteins in mixtures, identification of phosphorylation sites and other post translational modifications

Mass Spectrometry

core operated

Jordan

1105

Thermo TSQ Quantum Access

absolute quantitation of peptides and proteins with appropriate standards.

Triple quadrupole machine.

Mass Spectrometry

core operated

Jordan

1105

 

 

 

 

 

 

7 Tesla Bruker/Siemens ClinScan small animal MRI

MRI scans of small animals

Molecular Imaging Core

core or user operated

Snyder Building

 

4.7 Tesla Varian 200/400 MRI small animal imager with Varian Direct Drive imaging console

MRI scans of small animals

Molecular Imaging Core

core or user operated

MR4

 

Caliper IVIS Spectrum bioluminescence and fluorescence scanner

fluorescence and luminescence scanner for tissue samples

Molecular Imaging Core

core or user operated

Snyder Building

 

Siemens Medical Solutions Focus 120  Micro Positron Emission Tomography scanner

scan tissues or small animals using positrons

Molecular Imaging Core

 

Snyder Building

 

Siemens Eclipse  Cyclotron

production of isotopes for PET scanning

Molecular Imaging Core

core operated (Siemens/PETNET)

Snyder Building

 

Radiochemistry modules

synthesis of radiolabels

Molecular Imaging Core

core operated

Snyder Building

 

 

 

 

 

 

 

Tissue processors for formalin and Bouin's fixed tissues

 

Research Histology Core

core operated

MR4

1123

tissue embedding station

 

Research Histology Core

core operated

MR4

1123

microtomes

preparing thin sections for microscopy

Research Histology Core

core operated

MR4

1123

stainer, automated

staining of tissue sections

Research Histology Core

core operated

MR4

1123

cryostat

preparing frozen tissue sections for microscopy

Research Histology Core

core operated

MR4

1123

 

 

 

 

 

 

 

 

 

 

 

 

fluorescent plate reader

assays using fluorescent reagents in 6 to 384 well plates

Shared Instrumentation

user operated

Jordan

1074

circular dichroism spectropolarimeter, Aviv 410

secondary structure of proteins, including melting studies

Shared Instrumentation

user operated

Jordan

1074

Bio-Rad FX fluorescent gel scanner

scanning gels stained with Sypro Ruby and other fluorescent stains. 3 laser sources, several filters to cover common visible wavelengths.

Shared Instrumentation

core operated

Jordan

232

Dionex HPLC system for 0.3 to 4.6 mm diameter columns

analysis of small samples of  peptides, proteins and other molecules

Shared Instrumentation

core operated

Jordan

1051

Agilent HPLC system for 2 mm diameter columns, UV detector

reverse phase chromatography of small samples, manual fraction collection

Shared Instrumentation

core operated

Jordan

1051

Amersham FPLC

separation of proteins, peptides, and other molecules in aqueous buffers by gel filtration or ion exchange; a steel free system

Shared Instrumentation

core operated

Jordan

232

Bio-Rad GS-800 densitometer

scanning X-ray type films, gels stained with visible stains like Coomassie and silver

Shared Instrumentation

user operated

Jordan

1074

ImageQuant Tl software

relative quantitation of bands on gels and film

Shared Instrumentation

user operated

Jordan

1074

Aperio ScanScope

scanning microscopy slide to digital images at magnifications of 20X, 40X, 63X for digital pathology

Shared Instrumentation

user operated

Jordan

1074

Pharmacia/GE IPGphor isoelectric focusing apparatus, with 18 cm strip holders, Ettan Dalt 6 electrophoresis apparatus

isoelectric focusing

Shared Instrumentation

user operated

Jordan

232

Bio-Rad Protean IEF cell, 11 cm, 17 cm, 24 cm strip holders,Criterion Dodeca cell Protean II Xi cell,Protean Plus Dodeca cell

isoelectric focusing, 2-D gels

Shared Instrumentation

user operated

Jordan

232

Acea xCELLigence RTCA real time cell analysis instrument

real time, continuous measurement of cellular activity, including migration, based on impedance measurements

Shared Instrumentation

user operated

Jordan

1074

Biotek ELx800 absorbance plate reader

absorbance measurements on microtiter plates at 450, 562, 600, 660 nm

Shared Instrumentation

user operated

Jordan

1074

 

 

 

 

 

 

Inverted microscopes, Axio and Olympus

 

Tissue Culture Facility

core or user operated

Jordan

2-34

Olympus IM microscope

 

Tissue Culture Facility

core or user operated

Jordan

2-34

CO2 incubators

 

Tissue Culture Facility

core or user operated

Jordan

2-34

Incubators

maintenance of insect cell lines

Tissue Culture Facility

core operated

Jordan

2-34

cold storage, -80°C, liquid nitrogen

cell storage

Tissue Culture Facility

core operated

Jordan

2-34

Biosafety cabinets (3)

cell culture

Tissue Culture Facility

user operated

Jordan

2-34

 

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